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Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout
ACS Sensors ( IF 8.2 ) Pub Date : 2024-01-12 , DOI: 10.1021/acssensors.3c02486
Huabin Cai 1 , Yanling Huang 1 , Yue Lin 1 , Fang Luo 1 , Lifen Chen 2 , Longhua Guo 2 , Cuiying Lin 1 , Jian Wang 1 , Bin Qiu 1 , Zhenyu Lin 1
ACS Sensors ( IF 8.2 ) Pub Date : 2024-01-12 , DOI: 10.1021/acssensors.3c02486
Huabin Cai 1 , Yanling Huang 1 , Yue Lin 1 , Fang Luo 1 , Lifen Chen 2 , Longhua Guo 2 , Cuiying Lin 1 , Jian Wang 1 , Bin Qiu 1 , Zhenyu Lin 1
Affiliation
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Changes in the charge density on the inner surface of the microchannel can modulate the ion concentration at the tip, thus causing changes in the resistance of the system. In this study, this property is adopted to construct a portable sensor using a multimeter and aflatoxin B1 (AFB1) is used as the model target. Initially, the cDNA/aptamer complex is modified in the microchannel. The inner microchannel surface’s charge density is then altered by the recognition of the target, leading to a change in the system’s resistance, which can be conveniently monitored using a multimeter. Critical parameters influencing the performance of the system are optimized. Under optimum conditions, the resistance is linearly related to the logarithm of AFB1 concentration in the range of 100 fM–10 nM and the detection limit is 46 fM (S/N = 3). The resistive measurement is separated from the recognition reaction of the target, reducing the matrix interference during the detection process. This sensor boasts high sensitivity and specificity coupled with commendable reproducibility and stability. It is applied to assay the AFB1 content successfully in an actual sample of corn. Moreover, this approach is cost-effective, user-friendly, and highly accurate.
中文翻译：
基于万用表读数调节微通道电阻的便携式黄曲霉毒素 B1 传感器
微通道内表面电荷密度的变化可以调节尖端的离子浓度，从而引起系统电阻的变化。在本研究中，利用这一特性使用万用表构建便携式传感器，并使用黄曲霉毒素 B1 (AFB1) 作为模型目标。最初，cDNA/适体复合物在微通道中被修饰。然后，通过识别目标来改变内部微通道表面的电荷密度，从而导致系统电阻的变化，可以使用万用表方便地监测该变化。对影响系统性能的关键参数进行了优化。在最佳条件下，电阻与AFB1浓度的对数在100 fM–10 nM范围内呈线性相关，检测限为46 fM（ S / N = 3）。电阻测量与目标的识别反应分开，减少了检测过程中的基体干扰。该传感器具有高灵敏度和特异性以及值得称赞的再现性和稳定性。成功测定了玉米实际样品中AFB1的含量。此外，这种方法具有成本效益、用户友好且高度准确。
更新日期：2024-01-12
中文翻译：
基于万用表读数调节微通道电阻的便携式黄曲霉毒素 B1 传感器
微通道内表面电荷密度的变化可以调节尖端的离子浓度，从而引起系统电阻的变化。在本研究中，利用这一特性使用万用表构建便携式传感器，并使用黄曲霉毒素 B1 (AFB1) 作为模型目标。最初，cDNA/适体复合物在微通道中被修饰。然后，通过识别目标来改变内部微通道表面的电荷密度，从而导致系统电阻的变化，可以使用万用表方便地监测该变化。对影响系统性能的关键参数进行了优化。在最佳条件下，电阻与AFB1浓度的对数在100 fM–10 nM范围内呈线性相关，检测限为46 fM（ S / N = 3）。电阻测量与目标的识别反应分开，减少了检测过程中的基体干扰。该传感器具有高灵敏度和特异性以及值得称赞的再现性和稳定性。成功测定了玉米实际样品中AFB1的含量。此外，这种方法具有成本效益、用户友好且高度准确。
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