<br>基于转录组的分析揭示了 20E/HR3 通路在菜青虫滞育中的关键作用,Pesticide Biochemistry and Physiology

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基于转录组的分析揭示了 20E/HR3 通路在菜青虫滞育中的关键作用
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2024-01-11 , DOI: 10.1016/j.pestbp.2024.105787
Jing Liao ₁ , Dingxue Cai ₁ , Shaolei Geng ₂ , Zhaopeng Lyu ₁ , Yaling Wu ₁ , Jianjun Guo ₁ , Haiyin Li ₁

Affiliation

害虫是全球最具破坏性的害虫之一，滞育使其对环境压力具有很强的抵抗力，在生存和繁殖中发挥着至关重要的作用，同时也加剧了害虫管理和控制的挑战。然而，对其滞育调节的机制仍知之甚少。这项研究使用 RNA 测序来分析三个滞育阶段（诱导和准备、起始、维持）和同步非滞育阶段的转录组。在每个比较阶段，发现 759、1045 和 4721 个基因存在差异表达。其中，7个时钟基因和7个关键激素合成代谢基因被鉴定为在滞育型和非滞育型中具有差异表达模式。加权基因共表达网络分析（WGCNA）显示红色和蓝色模块对于滞育起始至关重要，而灰色模块被认为对于滞育维持至关重要。同时，在这些 hub 模块中鉴定出了 hub 基因、、等。此外，蜕皮激素下游核受体基因被发现是所有三个阶段的共享转录因子。 RNA 干扰导致蛹发育延迟，表明其参与调节蛹的滞育。进一步的激素测定显示，滞育型蛹中20-羟基蜕皮酮（20E）滴度低于非滞育型蛹，与相似。当将20E注射到滞育蛹中时，表达水平提高，并且蛹滞育被打破。这些结果表明20E/HR3途径是滞育调节的关键途径，通过蜕皮激素处理或RNAi干扰该途径将导致滞育的破坏。这些发现为滞育的分子机制提供了初步见解，并提出了专门针对滞育的蜕皮激素类似物和 RNAi 农药作为害虫防治手段的潜在用途。

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Transcriptome-based analysis reveals a crucial role of the 20E/HR3 pathway in the diapause of Pieris rapae

is among the most damaging pests globally, and diapause makes it highly resistant to environmental stresses, playing a crucial role in the survival and reproduction of while exacerbating the challenges of pest management and control. However, the mechanisms of its diapause regulation remain poorly understood. This research used RNA sequencing to profile the transcriptomes of three diapause phases (induction and preparation, initiation, maintenance) and synchronous nondiapause phases in . During each comparison phase, 759, 1045, and 4721 genes were found to be differentially expressed. Among these, seven clock genes and seven pivotal hormone synthesis and metabolism genes were identified as having differential expression patterns in diapause type and nondiapause type. The weighted gene co-expression network analysis (WGCNA) revealed the red and blue modules as pivotal for diapause initiation, while the grey module was identified to be crucial to diapause maintenance. Meanwhile, the hub genes , , , and so on, were identified within these hub modules. Moreover, an ecdysone downstream nuclear receptor gene, was found to be a shared transcription factor across all three phases. RNA interference of resulted in delayed pupal development, indicating its involvement in regulating pupal dipause in . The further hormone assays revealed that the 20-hydroxyecdysone (20E) titer in diapause type pupae was lower than that in nondiapause type pupae, which exhibited a similar trend to . When 20E was injected into diapause pupae, the expression levels were improved, and the pupal diapause were broken. These results indicate that the 20E/HR3 pathway is a critical pathway for the diapause regulation of and perturbing this pathway by ecdysone treatment or RNAi would result in the disruption of diapause. These findings provide initial insights into the molecular mechanisms of diapause and suggest the potential use of ecdysone analogs and RNAi pesticides, which specifically target to diapause, as a means of pest control in .

更新日期：2024-01-11

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