<br>改进的体外胃肠道消化方案模拟刷状缘消化，用于测定不同食物基质的可消化必需氨基酸评分 (DIAAS),Food Research International

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改进的体外胃肠道消化方案模拟刷状缘消化，用于测定不同食物基质的可消化必需氨基酸评分 (DIAAS)
Food Research International ( IF 7.0 ) Pub Date : 2024-01-11 , DOI: 10.1016/j.foodres.2024.113932
Delphine Martineau-Côté ₁ , Allaoua Achouri ₂ , Mélanie Pitre ₂ , Salwa Karboune ₃ , Lamia L'Hocine ₂

Affiliation

可消化必需氨基酸评分（DIAAS）是评估蛋白质营养质量的新金标准方法。 DIAAS 的评估模型复杂、受限且成本高昂。目前还没有既定的方法来评估它。在这项研究中，我们建议在国际胃肠道食物命运卓越网络（INFOGEST 方案）开发的标准化胃肠道消化方案中添加空肠-回肠消化阶段，以模拟刷状缘消化，并以更生理相关的方式进行 DIAAS 评估。该空肠-回肠消化阶段使用猪肠氨基肽酶进行，作为刷状缘膜提取物的替代品，后者更难以以标准化方式获得。这种修改后的 INFOGEST 方案适用于各种食品基质（蚕豆、豌豆和大豆粉、乳清分离蛋白和酪蛋白），并将结果与已发表的数据进行比较，以评估模型的生理相关性。空肠-回肠消化阶段的添加导致游离氨基酸和总氨基酸消化率分别显着增加 (< 0.05) 31% 和 29%，所有食物基质的 DIAAS 值平均增加 83%。尽管与 DIAAS 相比，DIAAS 仍然被低估，但观察到它们之间存在很强的相关性 (r = 0.879, = 0.009)，说明了最后一个消化阶段的相关性。当检测不适用时，建议将这种改进的消化方案作为评估 DIAAS 的合适替代方案。

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Improved in vitro gastrointestinal digestion protocol mimicking brush border digestion for the determination of the Digestible Indispensable Amino Acid Score (DIAAS) of different food matrices

The Digestible Indispensable Amino Acid Score (DIAAS) is the new gold standard method for the assessment of protein nutritional quality. The DIAAS is evaluated with models, that are complex, constraining and costly. There is still no established method to assess it In this study, we proposed to add a jejunal-ileal digestion phase to the standardized gastrointestinal digestion protocol developed by the International Network of Excellence on the Fate of Food in the Gastrointestinal Tract (INFOGEST protocol) to mimic brush border digestion and to enable DIAAS assessment in a more physiologically relevant manner. This jejunal-ileal digestion phase was performed with a porcine intestinal aminopeptidase as an alternative to brush border membrane extract, which is more difficult to obtain in a standardized way. This modified INFOGEST protocol was applied to various food matrices (faba bean, pea and soy flours, whey protein isolate and caseins) and the results were compared to published data to assess the model’s physiological relevance. The addition of the jejunal-ileal digestion phase lead to a significant ( < 0.05) increase of 31 and 29 % in free and total amino acid digestibility, respectively, and of 83 % on average for the DIAAS values for all food matrices. Although the DIAAS remained underestimated compared to the ones, a strong correlation between them was observed (r = 0.879, = 0.009), stating the relevance of this last digestion phase. This improved digestion protocol is proposed as a suitable alternative to evaluate the DIAAS when assays are not applicable.

更新日期：2024-01-11

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