South African Journal of Botany ( IF 2.7 ) Pub Date : 2024-01-10 , DOI: 10.1016/j.sajb.2023.12.015
Leyla Güven , Hasan Can , Adem Ertürk , Fatma Demirkaya Miloğlu , Mehmet Koca , Fatmanur İnce , İlhami Gülçin
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Thymus species (Lamiaceae), commonly referred to as thyme, have gained popularity due to their utilisation in traditional medicine for various ailments such as gastrointestinal disorders, colds, upper respiratory infections, vomiting, jaundice, oral hygiene and oral ulcers. Nevertheless, a significant number of Thymus species remain unexplored in terms of their bioactivity and phenolic content. In the present study, a thorough chemical analysis of endemic Thymus canoviridis methanol (METC)-water extracts (WETC) and Thymus pubescens var. pubescens methanol (METP)-water extracts (WETP) was carried out by Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry (UPLC-MS/MS). In addition, the inhibitory effect and antioxidant properties of these extracts on the enzymes acetylcholinesterase (AChE), α-glycosidase and carbonic anhydrase II (CA II) were also evaluated. The antioxidant potential was assessed using six assays, namely DPPH, ABTS, DMPD, FRAP, CUPRAC and Fe3+ reducing assays. Our results suggest that the methanol extract from these two plants exhibits a higher level of antioxidant activity. Furthermore, WETP inhibited all enzymes in the best way. According to the UPLC-MS/MS analysis results, rosmarinic acid showed its highest content in METC (18285.55 µg/g extract), WETC (27.98 µg/g extract) and METP (2910.59 µg/g extract), while WETP (69.76 µg/g extract) and the following total amounts of phenolic compounds were METC 20726.55 µg/g extract, METP 5887.20 µg/g extract, WETP 348.98 µg/g extract, WETC 68.56 µg/g extract. It is consistent with the analytical result, total phenolic content, and activity ranking in antioxidant assays. Regard to the results of the molecular docking studies, there is a high binding affinity between the CA II enzyme and cyanidin-3-O-glycoside, between the AChE and keracyanin chloride, and between α-glycosidase enzyme and rosmarinic acid. The extracts were discovered to exhibit various biological activities, such as antioxidant, antidiabetic, anti-Alzheimer's disease, and antiglaucoma properties.
中文翻译:
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百里香(地方性)和毛百里香变种的综合代谢分析。使用 UPLC-MS/MS 评估毛竹的抗氧化活性、酶抑制能力和分子对接研究
百里香属(唇形科)通常被称为百里香,因其在传统医学中用于治疗胃肠道疾病、感冒、上呼吸道感染、呕吐、黄疸、口腔卫生和口腔溃疡等各种疾病而受到欢迎。然而,大量百里香物种的生物活性和酚类含量仍未被探索。在本研究中,对地方性百里香甲醇(METC)-水提取物(WETC)和毛百里香变种进行了彻底的化学分析。采用超高效液相色谱串联质谱法 (UPLC-MS/MS) 分析毛竹甲醇 (METP)-水提取物 (WETP) 。此外,还评估了这些提取物对乙酰胆碱酯酶(AChE)、 α-糖苷酶和碳酸酐酶 II(CA II)的抑制作用和抗氧化特性。使用六种测定法评估抗氧化潜力,即 DPPH、ABTS、DMPD、FRAP、CUPRAC 和 Fe 3+还原测定法。我们的结果表明,这两种植物的甲醇提取物表现出更高水平的抗氧化活性。此外,WETP 以最佳方式抑制所有酶。根据UPLC-MS/MS分析结果,迷迭香酸在METC(18285.55 µg/g提取物)、WETC(27.98 µg/g提取物)和METP(2910.59 µg/g提取物)中含量最高,而WETP(69.76 µg/g提取物)中迷迭香酸含量最高。 /g提取物),酚类化合物总量如下:METC 20726.55 µg/g提取物、METP 5887.20 µg/g提取物、WETP 348.98 µg/g提取物、WETC 68.56 µg/g提取物。与抗氧化测定中的分析结果、总酚含量和活性排序一致。分子对接研究结果显示,CA II酶与花青素-3- O-糖苷、AChE与角花青素氯化物、α-糖苷酶与迷迭香酸之间具有较高的结合亲和力。这些提取物被发现具有多种生物活性,例如抗氧化、抗糖尿病、抗阿尔茨海默病和抗青光眼特性。