<br>光周期和外源褪黑激素对性成熟珍珠鸡（Numida meleagris）睾丸形态生理的影响,Animal Reproduction Science

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光周期和外源褪黑激素对性成熟珍珠鸡（Numida meleagris）睾丸形态生理的影响
Animal Reproduction Science ( IF 2.2 ) Pub Date : 2024-01-10 , DOI: 10.1016/j.anireprosci.2024.107410
Innocent J Gosomji ₁ , Umar M Bello ₂ , Tavershima Dzenda ₃ , Abdullahi Baso ₄ , Augustine Arukwe ₅ , Tom A Aire ₆

Affiliation

模拟光周期和褪黑激素对控制珍珠鸡繁殖的生物学效应尚不清楚。在此，三十 (30) 只性成熟的珍珠鸡被随机分配到 1-6 组 (n = 5)，并在存在或不存在外源褪黑激素（梅尔；1 毫克/公斤体重/天，i/ m) 八周。对安乐死公鸡的睾丸进行总体形态学、组织学、组织化学和氧化应激标记物的处理。使用酶联免疫吸附测定（ELISA）技术测定血清样品中的睾酮浓度。我们观察到长日照（LD）光周期下的梅尔组和非梅尔组以及短日照（SD）光周期下的非梅尔组睾丸大小有所增加。相反，SD 组的睾丸大小显着减小。 SD 的 Mel 组和非 Mel 组的生精小管显示出细胞形态学变化，包括细胞变性、局灶性空泡形成和生发上皮的耗竭。然而，LD 的 Mel 组和非 Mel 组的生发上皮似乎都是完整且活跃的。在所有组中，睾丸均显示出不同强度的 PAS 阳性染色。不同光周期方案和外源褪黑激素之间的PAS染色强度存在显着差异。该研究观察了光周期和外源性褪黑激素对谷胱甘肽还原酶（GSH）、丙二醛（MDA）和血清睾酮的相互作用。总体而言，结果表明，长日照（LD）光周期与外源褪黑激素相结合，通过增加睾丸大小和血清睾酮浓度来增强雄性珍珠鸡的繁殖活性。

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Influence of photoperiod and exogenous melatonin on testis morpho-physiology of sexually mature guinea fowl (Numida meleagris)

The biological effects of simulated photoperiod and melatonin on the control of reproduction of guinea fowls () are not well understood. Herein, thirty (30) sexually mature guinea fowl cocks were randomly assigned to 1–6 groups (n = 5) and subjected to different photoperiodic regimes in the presence or absence of exogenous melatonin (Mel; 1 mg/kgBW/day, i/m) for eight weeks. Testes of the euthanized cocks were processed for gross morphology, histological, histochemical, and oxidative stress markers. Testosterone concentration was determined in serum samples using the enzyme-linked immunosorbent assay (ELISA) technique. We observed an increase in testicular size in the Mel and Non-Mel groups under long-day (LD) photoperiods, and in the Non-Mel group under short-day (SD) photoperiod. Conversely, the testicular size was drastically reduced in the Mel group for SD. Seminiferous tubules in the Mel and Non-Mel groups of the SD showed cytomorphological changes, including degenerated cells, focal vacuolations, and depletion of germinal epithelium. However, the germinal epithelium appeared to be complete and active in both the Mel and Non-Mel groups for the LD. In all groups, the testes showed positive staining for PAS with varying intensities. There was a significant difference in PAS-staining intensity between different photoperiodic regimes and exogenous melatonin. The study observed the interaction between photoperiods and exogenous melatonin on glutathione reductase (GSH), malondialdehyde (MDA), and serum testosterone. Overall, the results indicated that a long-day (LD) photoperiod, combined with exogenous melatonin, enhanced reproductive activity in male guinea fowl by increasing testicular size and serum testosterone concentration.

更新日期：2024-01-10

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