Unexpected absence of exo-erythrocytic merogony during high gametocytaemia in two species of Haemoproteus (Haemosporida: Haemoproteidae), including description of Haemoproteus angustus n. sp. (lineage hCWT7) and a report of previously unknown residual bodies during in vitro gametogenesis,International Journal for Parasitology: Parasites and Wildlife

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Unexpected absence of exo-erythrocytic merogony during high gametocytaemia in two species of Haemoproteus (Haemosporida: Haemoproteidae), including description of Haemoproteus angustus n. sp. (lineage hCWT7) and a report of previously unknown residual bodies during in vitro gametogenesis
International Journal for Parasitology: Parasites and Wildlife ( IF 2.0 ) Pub Date : 2024-01-09 , DOI: 10.1016/j.ijppaw.2024.100905
Gediminas Valkiūnas ₁ , Tatjana Iezhova ₁ , Mikas Ilgūnas ₁ , Mélanie Tchoumbou ₁ , Mélanie Duc ₁ , Dovilė Bukauskaitė ₁ , Tanja Himmel ₂ , Josef Harl ₂ , Herbert Weissenböck ₂

Affiliation

Neglected avian blood parasites of the genus (Haemoproteidae) have recently attracted attention due to the application of molecular diagnostic tools, which unravelled remarkable diversity of their exo-erythrocytic (or tissue) stages both regarding morphology and organ tropism levels. The development of haemoproteids might result in pathologies of internal organs, however the exo-erythrocytic development (EED) of most species remains unknown. Seven individual birds - (1) and (6) – with high gametocytaemia (between 1% and 24%) of n. sp. (hCWT7) and (lineage hWW1) were sampled in Lithuania, and their internal organs were examined extensively by parallel application of histology and chromogenic in situ hybridization methods. Tissue stages were apparently absent, suggesting that the parasitaemia was not accompanied by detectable tissue merogony. n. sp. was described and characterized morphologically and molecularly. Sexual process and ookinete development of the new species readily occurred in vitro, and a unique character for parasites was discovered – the obligatory development of several tiny residual bodies, which were associated with intracellular transformation of both macrogametocytes and microgametocytes before their escape from the host cells and formation of gametes. A DNA haplotype network was constructed with lineages that cluster in one clade with the lineage hCWT7. This clade consists of lineages mostly found in birds, indicating specificity for birds of this genus. The lineage hCWT7 is mainly a parasite of Most reports of this lineage came from Turkey, with only a few records in Europe, mostly in birds wintering in Africa where transmission probably occurs. This study highlights unexpected difficulties in the research of EED even when using sensitive molecular diagnostic tools and extends information about transformation in early stages of gametogenesis in haemosporidian parasites.

中文翻译：

在两种 Haemoproteus（血孢虫纲：Haemoproteidae）的高配子细胞血症期间意外缺乏外红细胞分脉，包括 Haemoproteus angustus n. sp.（谱系 hCWT7）的描述和体外配子形成过程中先前未知的残留体的报告

由于分子诊断工具的应用，被忽视的禽血寄生虫属（Haemoproteidae）最近引起了人们的关注，这些工具揭示了它们在形态和器官嗜性水平方面的外红细胞（或组织）阶段的显着多样性。血蛋白的发育可能导致内脏器官的病理，但大多数物种的外红细胞发育（EED）仍然未知。在立陶宛对 7 只个体 - （1）和（6） - 患有高配子细胞血症（1% 至 24%）的 n. sp. （hCWT7）和（谱系 hWW1）进行了采样，并通过平行应用组织学和显色原位杂交方法广泛检查了它们的内脏。组织分期明显不存在，表明寄生虫血症不伴有可检测到的组织分叉。对 N. sp. 进行了形态学和分子学描述和表征。新物种的性过程和卵状发育很容易在体外发生，并发现了寄生虫的独特特征——几个微小残余体的强制性发育，这些残体与大配子细胞和微配子细胞在逃离宿主细胞和形成配子之前的细胞内转化有关。构建了一个 DNA 单倍型网络，其谱系与 hCWT7 谱系聚集在一个分支中。该分支由主要在鸟类中发现的谱系组成，表明对该属鸟类具有特异性。该谱系 hCWT7 主要是一种寄生虫该谱系的大多数报道来自土耳其，在欧洲只有少数记录，主要是在可能发生传播的非洲越冬的鸟类中。这项研究强调了即使在使用敏感的分子诊断工具时，EED 研究中也存在意想不到的困难，并扩展了有关血孢子虫寄生虫配子发生早期阶段转化的信息。

更新日期：2024-01-09

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