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A dual-mode lateral flow immunoassay by ultrahigh signal-to background ratio SERS probes for nitrofurazone metabolites ultrasensitive detection
Food Chemistry ( IF 8.5 ) Pub Date : 2024-01-09 , DOI: 10.1016/j.foodchem.2024.138374 Yanli Tian 1 , Xuechi Yin 1 , Jiawei Li 2 , Leina Dou 3 , Shaochi Wang 1 , Conghui Jia 1 , Yuechun Li 1 , Yaqian Chen 1 , Shengxue Yan 1 , Jianlong Wang 1 , Daohong Zhang 1
Food Chemistry ( IF 8.5 ) Pub Date : 2024-01-09 , DOI: 10.1016/j.foodchem.2024.138374 Yanli Tian 1 , Xuechi Yin 1 , Jiawei Li 2 , Leina Dou 3 , Shaochi Wang 1 , Conghui Jia 1 , Yuechun Li 1 , Yaqian Chen 1 , Shengxue Yan 1 , Jianlong Wang 1 , Daohong Zhang 1
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In this work, an ultra-sensitive lateral flow immunoassay (LFIA) with SERS/colorimetric dual signal mode was constructed for the detection of nitrofurazone metabolites, an antibiotic prohibited in animal-origin foods. Au@4-MBN@AgNRs nano-sandwich structural signal tag integrates the unique advantages of high signal-to-background ratio and anti-matrix interference through geometric control of SERS tag and nanoengineering adjustment of chemical composition. Under the optimal conditions, the detection limits of nitrofurazone metabolites by SERS/colorimetric dual-mode LFIA were 20 pg/mL (colorimetric mode) and 0.08 pg/mL (SERS mode). Excitingly, the vLOD of the colorimetric signal improved by a factor of 100 compared to Au NPs-based LFIA. In this study, the proposed dual-mode LFIA was successfully applied to the on-site real-time detection of honey, milk powder, and chicken. It is anticipated that with low background interference and anti-matrix interference output signal, our proposed dual-mode strategy can pave an innovative pathway for the fabrication of a powerful biosensor.
中文翻译：
采用超高信背景比 SERS 探针的双模式侧流免疫分析法，用于呋喃西林代谢物的超灵敏检测
在这项工作中，构建了一种具有 SERS/比色双信号模式的超灵敏侧流免疫分析 (LFIA)，用于检测呋喃西林代谢物，这是一种动物源性食品中禁用的抗生素。 Au@4-MBN@AgNRs纳米三明治结构信号标签通过SERS标签的几何控制和化学成分的纳米工程调节，融合了高信底比和抗基体干扰的独特优势。在最佳条件下，SERS/比色双模式LFIA对呋喃西林代谢物的检出限分别为20 pg/mL（比色模式）和0.08 pg/mL（SERS模式）。令人兴奋的是，与基于 Au NP 的 LFIA 相比，比色信号的 vLOD 提高了 100 倍。在本研究中，所提出的双模式LFIA成功应用于蜂蜜、奶粉和鸡肉的现场实时检测。预计，凭借低背景干扰和抗基质干扰输出信号，我们提出的双模式策略可以为制造强大的生物传感器铺平一条创新途径。
更新日期：2024-01-09
中文翻译：
采用超高信背景比 SERS 探针的双模式侧流免疫分析法，用于呋喃西林代谢物的超灵敏检测
在这项工作中，构建了一种具有 SERS/比色双信号模式的超灵敏侧流免疫分析 (LFIA)，用于检测呋喃西林代谢物，这是一种动物源性食品中禁用的抗生素。 Au@4-MBN@AgNRs纳米三明治结构信号标签通过SERS标签的几何控制和化学成分的纳米工程调节，融合了高信底比和抗基体干扰的独特优势。在最佳条件下，SERS/比色双模式LFIA对呋喃西林代谢物的检出限分别为20 pg/mL（比色模式）和0.08 pg/mL（SERS模式）。令人兴奋的是，与基于 Au NP 的 LFIA 相比，比色信号的 vLOD 提高了 100 倍。在本研究中，所提出的双模式LFIA成功应用于蜂蜜、奶粉和鸡肉的现场实时检测。预计，凭借低背景干扰和抗基质干扰输出信号，我们提出的双模式策略可以为制造强大的生物传感器铺平一条创新途径。
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