Doklady Biochemistry and Biophysics ( IF 0.8 ) Pub Date : 2024-01-08 , DOI: 10.1134/s1607672923700679 A A Zenchenko 1 , E M Savelieva 2 , M S Drenichev 1 , G A Romanov 2 , V E Oslovsky 1
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Abstract
For the first time, N6-(5-phenylpentan-1-yl)adenine, a synthetic adenine derivative with a receptor-specific anticytokinin effect, was obtained. This compound exhibits a pronounced anticytokinin effect, reducing cytokinin-induced expression of the GUS reporter gene when interacting with the cytokinin receptor CRE1/AHK4 of the model plant Arabidopsis thaliana. This effect manifests itself much weaker with the related AHK2 receptor and is not observed at all with the AHK3 receptor. We showed that N6-(5-phenylpentan-1-yl)adenine does not bind to the ligand-binding sites of the Arabidopsis cytokinin receptors, which does not allow it to be classified as a true cytokinin antagonist. Despite the currently unknown mechanism of action, this compound may find its use as a component of plant growth regulators. Like true anticytokinins, it enhances root growth of Arabidopsis seedlings, apparently suppressing the action of endogenous cytokinins on the “root” receptor CRE1/AHK4.
中文翻译：
N6-(5-苯基戊-1-基)腺嘌呤——一种新型非竞争性受体特异性抗细胞分裂素
抽象的
首次获得了具有受体特异性抗细胞分裂素作用的合成腺嘌呤衍生物N 6 -(5-苯基戊-1-基)腺嘌呤。该化合物表现出显着的抗细胞分裂素作用，当与模型植物拟南芥的细胞分裂素受体 CRE1/AHK4 相互作用时，可减少细胞分裂素诱导的GUS报告基因表达。这种效应在相关的 AHK2 受体中表现得较弱，而在 AHK3 受体中根本没有观察到这种效应。我们发现N 6 -(5-苯基戊-1-基)腺嘌呤不与拟南芥细胞分裂素受体的配体结合位点结合，这使得它不能被归类为真正的细胞分裂素拮抗剂。尽管目前尚不清楚其作用机制，但该化合物可能会被用作植物生长调节剂的成分。与真正的抗细胞分裂素一样，它可以促进拟南芥幼苗的根系生长，明显抑制内源性细胞分裂素对“根”受体 CRE1/AHK4 的作用。
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