is a traditional Chinese medicine for promoting blood circulation and eliminating blood stasis. Previous researches reported that Tanshinone IIA from had anti-angiogenic effect, however, whether its carbohydrates with similar effect is unknown. Here, we showed that a stachyose (Sta) from was purified by DEAE Sepharose Fast Flow anion-exchange chromatography and Bio-Gel P-2 gel permeation chromatography. Structure analyses suggested that Sta was tetrasaccharide as α-Gal-(1 → 6)-α-Gal-(1 → 6)-α-Glc-(1 → 2)-β-Fru. Further sulfated Sta was made and the degree of sulfate substitution was measured as 0.98. Bioactivity studies showed that Sta might block human microvascular endothelial cells tube formation and without significant cytotoxicity. Mechanism study suggested that Sta might bind to Galectin-3 (K: 5.777E) and inhibit its expression to block angiogenesis linked to VEGFR/FAK and PDGFR/AKT/ERK signaling. Evidence also supported that Sta might be a potential compound for developing stachyose as an anti-angiogenesis functional food.

中文翻译：

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水苏糖对云南丹参抗血管生成活性的影响

是活血化瘀的中药。既往研究报道丹参酮IIA具有抗血管生成作用，但其碳水化合物是否具有类似作用尚不清楚。在这里，我们展示了通过 DEAE Sepharose Fast Flow 阴离子交换色谱和 Bio-Gel P-2 凝胶渗透色谱纯化水苏糖 (Sta)。结构分析表明Sta为四糖α-Gal-(1→6)-α-Gal-(1→6)-α-Glc-(1→2)-β-Fru。进一步硫酸化Sta，测得硫酸酯取代度为0.98。生物活性研究表明，Sta可能会阻断人微血管内皮细胞管的形成，并且没有明显的细胞毒性。机制研究表明，Sta 可能与 Galectin-3 (K: 5.777E) 结合并抑制其表达，从而阻断与 VEGFR/FAK 和 PDGFR/AKT/ERK 信号传导相关的血管生成。证据还支持 Sta 可能是开发水苏糖作为抗血管生成功能食品的潜在化合物。