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Selection and Characterization of DNA Aptamers for Cytidine and Uridine
ChemBioChem ( IF 2.6 ) Pub Date : 2024-01-05 , DOI: 10.1002/cbic.202300656 Lide Gu 1, 2 , Jiajie Zheng 2 , Yao Zhang 1 , Deli Wang 1 , Juewen Liu 2
ChemBioChem ( IF 2.6 ) Pub Date : 2024-01-05 , DOI: 10.1002/cbic.202300656 Lide Gu 1, 2 , Jiajie Zheng 2 , Yao Zhang 1 , Deli Wang 1 , Juewen Liu 2
Affiliation
Using the library-immobilization capture-SELEX method, a highly selective DNA aptamer for cytidine was isolated with a Kd of 0.9 μM, whereas aptamers for uridine were of lower affinities. These aptamers do not bind to nucleobases or nucleotides and show good tolerance to metal ions.
中文翻译:
胞苷和尿苷 DNA 适体的选择和表征
使用文库固定化捕获-SELEX 方法,分离出高选择性的胞苷 DNA 适体, K d为 0.9 μM,而尿苷适体的亲和力较低。这些适体不与核碱基或核苷酸结合,并且对金属离子表现出良好的耐受性。
更新日期:2024-01-05
中文翻译:
胞苷和尿苷 DNA 适体的选择和表征
使用文库固定化捕获-SELEX 方法,分离出高选择性的胞苷 DNA 适体, K d为 0.9 μM,而尿苷适体的亲和力较低。这些适体不与核碱基或核苷酸结合,并且对金属离子表现出良好的耐受性。