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Magnetic microspheres enhanced peanut structure cascaded lasso shaped fiber laser biosensor for cancer marker-CEACAM5 detection in serum
Talanta ( IF 5.6 ) Pub Date : 2024-01-05 , DOI: 10.1016/j.talanta.2024.125625 Jie Hu 1 , Panpan He 2 , Fang Zhao 1 , Weihao Lin 1 , Chenlong Xue 1 , Jinna Chen 1 , Zhiguang Yu 2 , Yang Ran 3 , Yue Meng 4 , Xin Hong 5 , Perry Ping Shum 1 , Liyang Shao 1
Talanta ( IF 5.6 ) Pub Date : 2024-01-05 , DOI: 10.1016/j.talanta.2024.125625 Jie Hu 1 , Panpan He 2 , Fang Zhao 1 , Weihao Lin 1 , Chenlong Xue 1 , Jinna Chen 1 , Zhiguang Yu 2 , Yang Ran 3 , Yue Meng 4 , Xin Hong 5 , Perry Ping Shum 1 , Liyang Shao 1
Affiliation
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The detection of trace cancer markers in body fluids such as blood/serum is crucial for cancer diseases screening and treatment, which requires high sensitivity and specificity of biosensors. In this study, a peanut structure cascaded lasso (PSCL) shaped fiber sensing probe based on fiber laser demodulation method was proposed to specifically detect the carcinoembryonic antigen related cell adhesion molecules 5 (CEACAM5) protein in serum. Thanks for the narrow linewidth and high signal-to-noise ratio (SNR) of the laser spectrum, it is easier to distinguish small spectral changes than interference spectrum. Adding the antibody modified magnetic microspheres (MMS) to form the sandwich structure of "antibody-antigen-antibody-MMS", and amplified the response caused by biomolecular binding. The limit of detection (LOD) for CEACAM5 in buffer could reach 0.11 ng/mL. Considering the common threshold of 5 ng/mL for CEA during medical screening and the cut off limit of 2.5 ng/mL for some kits, the LOD of proposed biosensor meets the actual needs. Human serum samples from a hospital were used to validate the real sensing capability of proposed biosensor. The deviation between the measured value in various serum samples and the clinical value ranged from 1.9 to 9.8 %. This sensing scheme holds great potential to serve as a point of care testing (POCT) device and extend to more biosensing applications.
中文翻译：
磁性微球增强花生结构级联套索形光纤激光生物传感器用于血清中癌症标志物-CEACAM5检测
血液/血清等体液中痕量癌症标志物的检测对于癌症疾病的筛查和治疗至关重要，这需要生物传感器具有高灵敏度和特异性。本研究提出了一种基于光纤激光解调方法的花生结构级联套索（PSCL）形光纤传感探针，用于特异性检测血清中的癌胚抗原相关细胞粘附分子5（CEACAM5）蛋白。由于激光光谱具有窄线宽和高信噪比（SNR），因此比干涉光谱更容易区分微小的光谱变化。添加抗体修饰的磁性微球（MMS），形成“抗体-抗原-抗体-MMS”的三明治结构，放大生物分子结合引起的反应。缓冲液中 CEACAM5 的检测限 (LOD) 可达 0.11 ng/mL。考虑到医学筛查过程中 CEA 的常见阈值为 5 ng/mL，以及某些试剂盒的截止限值为 2.5 ng/mL，所提出的生物传感器的 LOD 满足实际需求。使用来自医院的人体血清样本来验证所提出的生物传感器的真实传感能力。各种血清样本的测量值与临床值的偏差范围为1.9%至9.8%。这种传感方案具有作为即时检测 (POCT) 设备并扩展到更多生物传感应用的巨大潜力。
更新日期：2024-01-05
中文翻译：
磁性微球增强花生结构级联套索形光纤激光生物传感器用于血清中癌症标志物-CEACAM5检测
血液/血清等体液中痕量癌症标志物的检测对于癌症疾病的筛查和治疗至关重要，这需要生物传感器具有高灵敏度和特异性。本研究提出了一种基于光纤激光解调方法的花生结构级联套索（PSCL）形光纤传感探针，用于特异性检测血清中的癌胚抗原相关细胞粘附分子5（CEACAM5）蛋白。由于激光光谱具有窄线宽和高信噪比（SNR），因此比干涉光谱更容易区分微小的光谱变化。添加抗体修饰的磁性微球（MMS），形成“抗体-抗原-抗体-MMS”的三明治结构，放大生物分子结合引起的反应。缓冲液中 CEACAM5 的检测限 (LOD) 可达 0.11 ng/mL。考虑到医学筛查过程中 CEA 的常见阈值为 5 ng/mL，以及某些试剂盒的截止限值为 2.5 ng/mL，所提出的生物传感器的 LOD 满足实际需求。使用来自医院的人体血清样本来验证所提出的生物传感器的真实传感能力。各种血清样本的测量值与临床值的偏差范围为1.9%至9.8%。这种传感方案具有作为即时检测 (POCT) 设备并扩展到更多生物传感应用的巨大潜力。
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