当前位置：
X-MOL 学术
›
Mar. Environ. Res.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Novel database for accA gene revealed a vertical variability pattern of autotrophic carbon fixation potential of ammonia oxidizing archaea in a permeable subterranean estuary
Marine Environmental Research ( IF 3.0 ) Pub Date : 2024-01-03 , DOI: 10.1016/j.marenvres.2024.106342 Yixiang Mao 1 , Jiapeng Wu 1 , Ruotong Yang 1 , Yuexi Ma 1 , Jiaqi Ye 1 , Jiarui Zhong 1 , Nanling Deng 1 , Xiang He 1 , Yiguo Hong 1
Marine Environmental Research ( IF 3.0 ) Pub Date : 2024-01-03 , DOI: 10.1016/j.marenvres.2024.106342 Yixiang Mao 1 , Jiapeng Wu 1 , Ruotong Yang 1 , Yuexi Ma 1 , Jiaqi Ye 1 , Jiarui Zhong 1 , Nanling Deng 1 , Xiang He 1 , Yiguo Hong 1
Affiliation
|
The autotrophic carbon fixation pathway of ammonia-oxidizing archaea (AOA) was the 3-hydroxypropionate/4-hydroxybutyrate (3-HP/4-HB) cycle, of which the acetyl-CoA carboxylase α-submit () gene is widely recognized as the indicator. To date, there is no reference database or suitable cut-off value for operational taxonomic unit (OTU) clustering to analyze the diversity of AOA based on the gene. In this study, a reference database with 489 sequences was constructed, all the gene sequences was obtained from the AOA enrichment culture, pure culture and environmental samples. Additionally, the 79% was determined as the cut-off value for OTU clustering by comparing the similarity between the gene and the 16S rRNA gene. The developed method was verified by analyzing samples from the subterranean estuary and a vertical variation pattern of autotrophic carbon fixation potential of AOA was revealed. This study provided an effective method to analyze the diversity and autotrophic carbon fixation potential of AOA based on gene.
中文翻译：
accA基因的新数据库揭示了可渗透的地下河口氨氧化古菌自养碳固定潜力的垂直变异模式
氨氧化古菌（AOA）的自养碳固定途径是3-羟基丙酸/4-羟基丁酸（3-HP/4-HB）循环，其中乙酰辅酶A羧化酶α-submit（）基因被广泛认为是指标。迄今为止，还没有参考数据库或合适的操作分类单元（OTU）聚类的截止值来分析基于基因的AOA多样性。本研究构建了包含489条序列的参考数据库，所有基因序列均来自AOA富集培养物、纯培养物和环境样本。另外，通过比较基因与16S rRNA基因之间的相似性，将79%确定为OTU聚类的截止值。通过分析地下河口的样品验证了所开发的方法，并揭示了 AOA 自养碳固定潜力的垂直变化模式。本研究为基于基因分析AOA多样性和自养固碳潜力提供了有效方法。
更新日期：2024-01-03
中文翻译：
accA基因的新数据库揭示了可渗透的地下河口氨氧化古菌自养碳固定潜力的垂直变异模式
氨氧化古菌（AOA）的自养碳固定途径是3-羟基丙酸/4-羟基丁酸（3-HP/4-HB）循环，其中乙酰辅酶A羧化酶α-submit（）基因被广泛认为是指标。迄今为止，还没有参考数据库或合适的操作分类单元（OTU）聚类的截止值来分析基于基因的AOA多样性。本研究构建了包含489条序列的参考数据库，所有基因序列均来自AOA富集培养物、纯培养物和环境样本。另外，通过比较基因与16S rRNA基因之间的相似性，将79%确定为OTU聚类的截止值。通过分析地下河口的样品验证了所开发的方法，并揭示了 AOA 自养碳固定潜力的垂直变化模式。本研究为基于基因分析AOA多样性和自养固碳潜力提供了有效方法。
京公网安备 11010802027423号