<br>中华根瘤菌的不同反应。暴露于 Pb 和 Zn 后：矿化与络合,Environmental Pollution

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中华根瘤菌的不同反应。暴露于 Pb 和 Zn 后：矿化与络合
Environmental Pollution ( IF 7.6 ) Pub Date : 2023-12-29 , DOI: 10.1016/j.envpol.2023.123260
Peihuan Li ₁ , Jiale Chen ₁ , Shumin Ying ₁ , Nike Chen ₁ , Shu Fang ₁ , Menglei Ye ₁ , Chunhua Zhang ₂ , Chonghua Li ₃ , Ying Ge ₁

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铅（Pb）和锌（Zn）已被排放到环境中，可能对生态安全产生负面影响。根瘤菌因其参与金属污染土壤的修复而受到关注。然而，人们对根瘤菌在铅和锌胁迫下的反应知之甚少，特别是胞外聚合物（EPS）在这两种金属的抵抗力中所发挥的作用。在这里，sp。 C10 是从矿区周围的土壤中分离出来的，并经过一系列的 Pb/Zn 处理。测定细胞形态和表面矿物质晶体、EPS含量和荧光物质。此外，还通过衰减全反射红外光谱（ATR-IR）和X射线光电子能谱（XPS）对胞外多糖和蛋白质进行了表征。结果表明，Zn胁迫诱导C10细胞合成EPS。多糖 (CO) 和蛋白质 (C-O/C-N) 的官能团参与与 Zn 的络合。相比之下，C10 通过在细胞表面形成磷酸铅 (Pb(PO)) 来抵抗 Pb 应力。半乳糖（Gal）和酪氨酸在抵抗锌毒性方面发挥着关键作用，而葡萄糖胺（N-Glc）在细胞外铅沉淀过程中大量转化为葡萄糖。总之，本研究表明C10对两种金属具有不同的解毒策略，可为Pb和Zn污染场地的生物修复提供依据。

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Different responses of Sinorhizobium sp. upon Pb and Zn exposure: Mineralization versus complexation

Lead (Pb) and zinc (Zn) have been discharged into environment and may negatively impact ecological security. Rhizobia has gained attention due to their involvement in the restoration of metal polluted soils. However, little is known about the responses of rhizobia under Pb and Zn stress, especially the roles played by extracellular polymeric substances (EPS) in the resistance of these two metals. Here, sp. C10 was isolated from soil around a mining area and was exposed to a series of Pb/Zn treatments. The cell morphology and surface mineral crystals, EPS content and fluorescent substances were determined. In addition, the extracellular polysaccharides and proteins were characterized by attenuated total reflection infrared spectroscopy (ATR-IR) and X-ray photoelectron spectroscopy (XPS). The results showed that Zn stress induced the synthesis of EPS by C10 cells. Functional groups of polysaccharides (CO) and proteins (C–O/C–N) were involved in complexation with Zn. In contrast, C10 resisted Pb stress by forming lead phosphate (Pb(PO)) on the cell surface. Galactose (Gal) and tyrosine played key roles in resistance to the Zn toxicity, whereas glucosamine (N-Glc) was converted to glucose in large amounts during extracellular Pb precipitation. Together, this study demonstrated that C10 possessed different strategies to detoxify the two metals, and could provide basis for bioremediation of Pb and Zn polluted sites.

更新日期：2023-12-29

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