<br>聚苯乙烯微塑料和 3,6-二溴咔唑对斑马鱼 (Danio rerio) 胚胎的联合毒性作用,Science of the Total Environment

当前位置： X-MOL 学术 › Sci. Total Environ. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

聚苯乙烯微塑料和 3,6-二溴咔唑对斑马鱼 (Danio rerio) 胚胎的联合毒性作用
Science of the Total Environment ( IF 8.2 ) Pub Date : 2024-01-04 , DOI: 10.1016/j.scitotenv.2023.169787
Jie Zhang ₁ , Yao Bai ₁ , Haoran Meng ₁ , Yangzhe Zhu ₁ , Huizhu Yue ₁ , Bing Li ₁ , Jinhua Wang ₁ , Jun Wang ₁ , Lusheng Zhu ₁ , Zhongkun Du ₁

Affiliation

微塑料（MPs）和多卤咔唑（PHCZs）在水环境中广泛检测，其生态风险已成为研究热点。尽管 MP 和 PHCZ 广泛共同分布，但它们对水生生物的综合毒性仍不清楚。本研究通过单独/联合暴露研究了聚苯乙烯微塑料 (PS-MP) 和 3,6-二溴咔唑 (3,6-DBCZ) 对斑马鱼胚胎的毒性作用。本研究表明，单独或联合暴露PS-MPs（10 mg/L）和3,6-DBCZ（0.5 mg/L）可显着增加斑马鱼胚胎畸形率，但对死亡率和孵化率没有显着影响。速度。此外，暴露于 3,6-DBCZ 或 PS-MP 会增加斑马鱼胚胎中活性氧 (ROS) 的水平，由此产生的氧化应激会诱导细胞凋亡。相比之下，联合暴露 3,6-DBCZ 和 PS-MP 可以显着降低斑马鱼胚胎中的氧化应激和细胞凋亡水平。这些观察结果表明，3,6-DBCZ 和 PS-MP 的联合暴露对氧化应激和细胞凋亡具有拮抗作用。荧光PS-MPs追踪和3,6-DBCZ富集分析表明，在绒毛膜的保护下，PS-MPs（5和50μm）进入斑马鱼胚胎阶段（55hpf）被阻止。此外，暴露96-144 hpf后，PS-MPs作为载体，通过摄入促进斑马鱼幼虫体内3,6-DBCZ的积累及其二恶英样毒性。与5μm PS-MPs相比，50μm PS-MPs促进斑马鱼幼虫中3,6-DBCZ更高的积累和二恶英样毒性。这些发现表明MP可以作为PHCZ的重要载体，影响其毒性和在生物体中的生物累积。

"点击查看英文标题和摘要"

Combined toxic effects of polystyrene microplastics and 3,6-dibromocarbazole on zebrafish (Danio rerio) embryos

Microplastics (MPs) and polyhalogenated carbazoles (PHCZs) are widely detected in the aquatic environment, and their ecological risks have become a research focus. Although there is an extensive co-distribution of MPs and PHCZs, their combined toxicity to aquatic organisms is still unclear. This study investigated the toxic effects of polystyrene microplastics (PS-MPs) and 3,6-dibromocarbazole (3,6-DBCZ) on zebrafish embryos by individual/combined exposure. This study showed that individual or combined exposure of PS-MPs (10 mg/L) and 3,6-DBCZ (0.5 mg/L) could significantly increase the rate of zebrafish embryo deformity, whereas no significant effect was observed on mortality and hatching rate. Furthermore, exposure to 3,6-DBCZ or PS-MPs increased reactive oxygen species (ROS) levels in zebrafish embryos, and the resulting oxidative stress induced apoptosis. Comparably, the levels of oxidative stress and apoptosis in zebrafish embryos were significantly reduced with the combined exposure of 3,6-DBCZ and PS-MPs. These observations suggest that the combined exposure of 3,6-DBCZ and PS-MPs has an antagonistic effect on oxidative stress and apoptosis. Fluorescence PS-MPs tracing and 3,6-DBCZ enrichment analysis showed that, with the protection of chorion, the entry of PS-MPs (5 and 50 μm) into the embryonic stage (55 hpf) of zebrafish was prevented. Moreover, after exposure for 96–144 hpf, PS-MPs serve as a carrier to promote the 3,6-DBCZ accumulation and its dioxin-like toxicity in zebrafish larvae through ingestion. Compared with 5-μm PS-MPs, 50-μm PS-MPs promoted higher accumulation and dioxin-like toxicity of 3,6-DBCZ in zebrafish larvae. These findings provide that MPs can be used as an important carrier of PHCZs, influencing their toxicity and bioaccumulation in the organisms.

更新日期：2024-01-04

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南

全部期刊列表>>