<br>Hippo/YAP1通过激活Wnt信号通路促进骨质疏松小鼠骨缺损修复,Cellular Signalling

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Hippo/YAP1通过激活Wnt信号通路促进骨质疏松小鼠骨缺损修复
Cellular Signalling ( IF 4.4 ) Pub Date : 2024-01-04 , DOI: 10.1016/j.cellsig.2024.111037
Kehan Li ₁ , Linan Liu ₂ , Hanghang Liu ₂ , Yao Liu ₂ , Jiawei Xing ₂ , Jian Song ₂ , En Luo ₂

Affiliation

本研究旨在探讨Hippo/YAP1在体内和体外修复老年小鼠骨质疏松性骨缺损中的作用和机制。我们在体内和体外研究了年轻人和老年人中 Hippo 信号传导的表达差异。通过操纵和的表达，我们研究了 Hippo/YAP1 在调节衰老 BMSC 成骨分化中的作用。在体内，通过分别干预和的局部和全身表达，我们试图证明 Hippo/YAP1 是否促进老年骨质疏松条件下骨缺损的修复。最后，我们深入研究了Hippo/YAP1调节成骨分化的潜在机制。我们观察到年轻人和老年人之间河马信号表达的差异。在老化的BMSCs中敲除后，我们观察到内源性YAP1的上调促进了细胞的成骨分化和增殖能力。通过干扰表达，我们为 Hippo/YAP1 在促进衰老 BMSC 成骨分化中的作用提供了有力的证据。在体内，我们证实Hippo/YAP1促进衰老骨质疏松症中骨缺损的修复。此外，我们还发现了 YAP1、β-catenin 和 TEAD1 之间的相互作用关系。本研究阐明了 Hippo/YAP1 在促进老年小鼠骨质疏松性骨缺损修复中的作用。从机制上讲，YAP1 通过激活 Wnt/β-catenin 通路发挥作用，并且该过程并不独立于 TEAD1。

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Hippo/YAP1 promotes osteoporotic mice bone defect repair via the activating of Wnt signaling pathway

This study is to investigate the role and mechanism of Hippo/YAP1 in the repair of osteoporotic bone defects in aged mice, both in vivo and in vitro. We investigated the expression differences of the Hippo signaling in young and aged individuals both in vivo and in vitro. By manipulating the expression of and , we investigated the role of Hippo/YAP1 in regulating osteogenic differentiation in aged BMSCs. In vivo, by intervening in the local and systemic expression of and respectively, we sought to demonstrate whether Hippo/YAP1 promotes the repair of bone defects in aged osteoporotic conditions. Finally, we delved into the underlying mechanisms of Hippo/YAP1 in regulating osteogenic differentiation. We observed differences in the expression of the Hippo signaling between young and aged individuals. After knocking out in aged BMSCs, we observed that the upregulation of endogenous YAP1 promotes cellular osteogenic differentiation and proliferation capacity. Through interference with expression, we provided strong evidence for the role of Hippo/YAP1 in promoting osteogenic differentiation in aged BMSCs. In vivo, we confirmed that Hippo/YAP1 promotes the repair of bone defects in aging osteoporosis. Moreover, we discovered an interaction relationship among YAP1, β-catenin, and TEAD1. This study elucidates the role of Hippo/YAP1 in promoting the repair of osteoporotic bone defects in aged mice. Mechanistically, YAP1 functions by activating the Wnt/β-catenin pathway, and this process is not independent of TEAD1.

更新日期：2024-01-04

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