Biosynthesis of 10-Hydroxy-2-decenoic Acid through a One-Step Whole-Cell Catalysis,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Biosynthesis of 10-Hydroxy-2-decenoic Acid through a One-Step Whole-Cell Catalysis
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-01-04 , DOI: 10.1021/acs.jafc.3c08142
Ke Fang _{1,

2} , Ziting Xu _{1,

2} , Lu Yang _{1,

2} , Quan Cui _{1,

2} , Bowen Du _{1,

2} , Huijing Liu _{1,

2} , Ruiming Wang _{1,

2} , Piwu Li _{1,

2} , Jing Su _{1,

2} , Junqing Wang _{1,

2}

Affiliation

10-Hydroxy-2-decenoic acid (10-HDA) is an important component of royal jelly, known for its antimicrobial, anti-inflammatory, blood pressure-lowering, and antiradiation effects. Currently, 10-HDA biosynthesis is limited by the substrate selectivity of acyl-coenzyme A dehydrogenase, which restricts the technique to a two-step process. This study aimed to develop an efficient and simplified method for synthesizing 10-HDA. In this study, ACOX from Candida tropicalis 1798, which catalyzes 10-hydroxydecanoyl coenzyme A desaturation for 10-HDA synthesis, was isolated and heterologously coexpressed with FadE, Macs, YdiI, and CYP in Escherichia coli/SK after knocking out FadB, FadJ, and FadR genes. The engineered E. coli/AKS strain achieved a 49.8% conversion of decanoic acid to 10-HDA. CYP expression was improved through ultraviolet mutagenesis and high-throughput screening, increased substrate conversion to 75.6%, and the synthesis of 10-HDA was increased to 0.628 g/L in 10 h. This is the highest conversion rate and product concentration achieved in the shortest time to date. This study provides a simple and efficient method for 10-HDA biosynthesis and offers an effective method for developing strains with high product yields.

中文翻译：

通过一步全细胞催化生物合成 10-羟基-2-癸烯酸

10-羟基-2-癸烯酸（10-HDA）是蜂王浆的重要成分，以其抗菌、抗炎、降血压和抗辐射作用而闻名。目前，10-HDA 生物合成受到酰基辅酶 A 脱氢酶的底物选择性的限制，这将该技术限制为两步过程。本研究旨在开发一种高效且简化的 10-HDA 合成方法。在本研究中，分离了来自热带假丝酵母1798的 ACOX，该 ACOX 催化 10-羟基癸酰辅酶 A 去饱和以合成 10-HDA，在敲除 FadB、FadJ、和 FadR 基因。工程化的大肠杆菌/AKS 菌株将癸酸转化为 10-HDA，转化率为 49.8%。通过紫外诱变和高通量筛选，提高CYP表达量，底物转化率提高至75.6%，10 h内10-HDA合成量提高至0.628 g/L。这是迄今为止在最短的时间内实现的最高转化率和产品浓度。本研究为10-HDA生物合成提供了一种简单高效的方法，为开发高产菌株提供了有效的方法。

更新日期：2024-01-04

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南