RNA Interference-Screening of Potentially Lethal Gene Targets in the White-Backed Planthopper Sogatella furcifera via a Spray-Induced and Nanocarrier-Delivered Gene Silencing System,Journal of Agricultural and Food Chemistry

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RNA Interference-Screening of Potentially Lethal Gene Targets in the White-Backed Planthopper Sogatella furcifera via a Spray-Induced and Nanocarrier-Delivered Gene Silencing System
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-01-03 , DOI: 10.1021/acs.jafc.3c05659
Yun-Feng Ma ₁ , Ting-Ting Liu ₂ , Ya-Qin Zhao ₁ , Juan Luo ₁ , Hong-Yan Feng ₁ , Yang-Yuntao Zhou ₁ , Lang-Lang Gong ₁ , Meng-Qi Zhang ₁ , Yin-Yin He ₁ , J Joe Hull ₃ , Youssef Dewer ₄ , Ming He ₁ , Peng He ₁

Affiliation

RNA interference (RNAi) is a widespread post-transcriptional silencing mechanism that targets homologous mRNA sequences for specific degradation. An RNAi-based pest management strategy is target-specific and considered a sustainable biopesticide. However, the specific genes targeted and the efficiency of the delivery methods can vary widely across species. In this study, a spray-induced and nanocarrier-delivered gene silencing (SI-NDGS) system that incorporated gene-specific dsRNAs targeting conserved genes was used to evaluate phenotypic effects in white-backed planthopper (WBPH). At 2 days postspraying, transcript levels for all target genes were significantly reduced and knockdown of two gene orthologs, hsc70-3 and PP-α, resulted in an elevated mortality (>60%) and impaired ecdysis. These results highlight the utility of the SI-NDGS system for identifying genes involved in WBPH growth and development that could be potentially exploitable as high mortality target genes to develop an alternative method for WBPH control.

中文翻译：

通过喷雾诱导和纳米载体传递的基因沉默系统对白背飞虱白背飞虱中潜在致死基因靶标进行 RNA 干扰筛选

RNA 干扰 (RNAi) 是一种广泛存在的转录后沉默机制，针对同源 mRNA 序列进行特异性降解。基于 RNAi 的害虫管理策略具有针对性，被认为是一种可持续的生物农药。然而，不同物种的具体靶向基因和递送方法的效率可能存在很大差异。在这项研究中，采用喷雾诱导和纳米载体传递的基因沉默（SI-NDGS）系统，该系统结合了针对保守基因的基因特异性双链RNA，用于评估白背飞虱（WBPH）的表型效应。喷洒后 2 天，所有目标基因的转录水平均显着降低，并且两个基因直系同源物hsc70-3和PP-α的敲低导致死亡率升高（> 60%）和蜕皮受损。这些结果强调了 SI-NDGS 系统在识别与 WBPH 生长和发育有关的基因方面的实用性，这些基因可能可用作高死亡率目标基因，以开发一种控制 WBPH 的替代方法。

更新日期：2024-01-03

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