<br>白斑综合症病毒（WSSV）在印度白对虾野生产卵种群中的垂直传播和流行,Journal of Invertebrate Pathology

当前位置： X-MOL 学术 › J. Invertebr. Pathol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

白斑综合症病毒（WSSV）在印度白对虾野生产卵种群中的垂直传播和流行
Journal of Invertebrate Pathology ( IF 3.6 ) Pub Date : 2024-01-03 , DOI: 10.1016/j.jip.2024.108058
K K Vijayan ₁ , P S Shyne Anand ₁ , C P Balasubramanian ₁ , Joseph Sahaya Rajan ₁ , P Ezhil Praveena ₁ , R Aravind ₁ , N S Sudheer ₁ , Biju Francis ₁ , A Panigrahi ₁ , S K Otta ₁

Affiliation

白斑病是由白斑综合症病毒（WSSV）引起的，历来是全世界对虾养殖业最具破坏性的疾病。病毒传播方式是病毒感染动态和管理中最关键的阶段。本研究利用实时定量 PCR (q RT PCR)、光学和电子显微镜以及杂交技术，探讨了 WSSV 在印度白虾（潜在的本地驯化和遗传改良物种）中垂直传播的机制。 2016 年至 2021 年期间，对印度东南海岸的野生育雏鸡（n = 2576）进行了采样。其中约 58% 的育雏鸡呈 WSSV 阳性，几乎 50% 的受感染野生育雏鸡处于感染的不同阶段。生殖成熟。对 WSSV-PCR 阳性育雏鸡 (n = 200) 进行了 WSSV 垂直传播分析。生殖组织的 q RT PCR 研究表明，61% (n = 13) 的精囊、54% (n = 28) 的未成熟卵巢和 48% (n = 27) 的成熟卵巢感染了 WSSV。卵巢成熟的雌性（6.84 × 10 ± 9.79 × 10 ng 基因组 DNA）中感染水平最低，其次是受精卵（1.59 × 10 ± 3.69 × 10 ng 基因组 DNA）和幼虫（无节幼体和幼虫）。具有高 WSSV 拷贝的妊娠雌性的组织学显示固缩和核破碎的生发囊泡具有退化的皮质杆。相反，WSSV 拷贝数低的妊娠雌性显示卵巢发育完全，没有 WSSV 感染的特征性迹象。透射电子显微镜研究清楚地证实了卵巢和精囊中存在 WSSV 颗粒。当进行杂交时，尽管妊娠卵巢和受精卵未能产生 WSSV 特异性信号，但在精囊结缔组织中观察到 WSSV 特异性信号。本研究为 WSSV 的卵巢垂直传播提供了第一个分子和组织学证据。无病基础群体的发展是建立育种计划的基石和第一步，目前的研究结果可以作为制定此类计划的基础。

"点击查看英文标题和摘要"

Vertical transmission and prevalence of white spot syndrome virus (WSSV) in the wild spawning population of the Indian white shrimp, Penaeus indicus

White spot disease, caused by white spot syndrome virus (WSSV), has historically been the most devastating disease in shrimp aquaculture industry across the world. The mode of virus transmission is the most crucial stage in the dynamics and management of virus infection. This study explored the mechanism of vertical transmission of WSSV in Indian white shrimp, , potential native species for domestication and genetic improvement, using quantitative real time PCR (q RT PCR), light and electron microscopy, and hybridization. Wild brooders of (n = 2576) were sampled along the South east coast of India, during 2016 to 2021. Of these ∼ 58 % of the brooders were positive for WSSV, and almost 50 % of infected wild brooders were at the various stages of reproductive maturation. WSSV-PCR positive brooders (n = 200) were analysed for vertical WSSV transmission. The q RT PCR studies of reproductive tissues revealed that 61 % (n = 13) of spermatophore, 54 % (n = 28) of immature ovaries and 48 % (n = 27) of ripe ovaries were infected with WSSV. The lowest level of infection was recorded in females with ripe ovaries (6.84 × 10 ± 9.79 × 10 ng genomic DNA) followed by fertilized eggs (1.59 × 10 ± 3.69 × 10 ng genomic DNA), and larvae (nauplius and zoea). The histology of gravid females with high WSSV copies showed pyknotic and karyorrhectic germinal vesicle with degenerated cortical rods. Conversely, the gravid females with low WSSV copies showed fully developed ovary without characteristic signs of WSSV infection. Transmission electron microscopic studies clearly established the presence of WSSV particles in both ovaries and spermatophores. When subjected to hybridization, WSSV-specific signals were observed in connective tissues of spermatophore, although gravid ovary and fertilized eggs were failed to produce WSSV specific signals. The present study provides the first molecular and histological evidence for -ovarian vertical transmission of WSSV. Development of disease-free base population being the cornerstone and first step in establishing the breeding program, the present findings could be a basis for development of such programs.

更新日期：2024-01-03

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南