Antibiotic altered liver damage induced by aflatoxin B1 exposure in mice by modulating the gut microbiota,Environmental Pollution

当前位置： X-MOL 学术 › Environ. Pollut. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Antibiotic altered liver damage induced by aflatoxin B1 exposure in mice by modulating the gut microbiota
Environmental Pollution ( IF 7.6 ) Pub Date : 2024-01-02 , DOI: 10.1016/j.envpol.2024.123291
Yongli Ye ₁ , Tingwei Wang ₂ , Jia-Sheng Wang ₃ , Jian Ji ₁ , Xiao Ning ₄ , Xiulan Sun ₁

Affiliation

Aflatoxins B1 (AFB1) and antibiotic (AN) carry co-exposure risks, with the gut being a target organ for their combined effects. However, the current understanding of the impact of AN on gut and liver injury induced by AFB1 remains limited. In this study, we conducted a 9-week investigation into the implications of AN (ampicillin and penicillin) treatment on AFB1-induced intestinal and liver injury in C57BL/6J male mice fed a normal diet (ND) and a high-fat diet (HFD). The results showed that AN treatment significantly reduce the total number and diversity of intestinal species in both ND and HFD mice exposed to AFB1. Moreover, AN treatment alleviated AFB1-induced liver injury and lipid accumulation in mice on ND and HFD, while improving abnormal lipid metabolism in the liver and serum. However, AN treatment also promoted intestinal damage and reduced the levels of short-chain fatty acids in the gut. Correlation analysis demonstrated that, under the two dietary patterns, microorganisms across various genera were significantly positively or negatively correlated with alterations in liver, serum, and intestinal biochemical indexes. These genera include , , , , and , . AN may alleviate long-term AFB1-induced liver injury through the regulation of intestinal microorganisms, with the effect being more pronounced in mice following an HFD pattern. These findings provide novel insights into the effects of AFB1 on the gut‒liver axis under complex exposure conditions, as well as the relationship between gut microbial homeostasis and liver injury across different dietary patterns.

中文翻译：

抗生素通过调节肠道微生物群改变黄曲霉毒素 B1 暴露引起的小鼠肝损伤

黄曲霉毒素 B1 (AFB1) 和抗生素 (AN) 具有共同暴露风险，肠道是其综合作用的靶器官。然而，目前对 AN 对 AFB1 引起的肠道和肝脏损伤的影响的了解仍然有限。在这项研究中，我们进行了为期 9 周的研究，研究 AN（氨苄西林和青霉素）治疗对正常饮食 (ND) 和高脂饮食 (ND) 喂养的 C57BL/6J 雄性小鼠 AFB1 诱导的肠道和肝脏损伤的影响。高频FD）。结果表明，AN 处理显着降低了暴露于 AFB1 的 ND 和 HFD 小鼠肠道物种的总数和多样性。此外，AN治疗减轻了ND和HFD小鼠中AFB1引起的肝损伤和脂质积累，同时改善了肝脏和血清中的异常脂质代谢。然而，AN 治疗也会促进肠道损伤并降低肠道中短链脂肪酸的水平。相关分析表明，两种膳食模式下，各属微生物与肝脏、血清、肠道生化指标的变化均呈显着正相关或负相关。这些属包括、、、、和、。 AN 可能通过调节肠道微生物来减轻 AFB1 引起的长期肝损伤，这种效果在遵循 HFD 模式的小鼠中更为明显。这些发现为复杂暴露条件下 AFB1 对肠肝轴的影响，以及不同饮食模式下肠道微生物稳态与肝损伤之间的关系提供了新的见解。

更新日期：2024-01-02

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南