Producing algae biomass economically remains one of the chief bottlenecks for commercializing algae products. The aim of this work is to identify new strains of algae that can grow on cost effective media derived from cellulosic waste streams, characterize the potential of these strains to produce compounds of high industrial value, and identify those strains capable of facile genetic transformation. Here we report, out of 45 strains initially isolated, three were selected based on their ability to efficiently grow on organic waste material (corn stover hydrolysate) as a carbon source; Chlorococcum sp., Desmodesmus sp., and Chlamydomonas debaryana. Untargeted metabolomics was performed on each strain, identifying several metabolites of high relative abundances that are of commercial interest, such as lactic acid, butane-2,3-diol, amino acids, tartaric acid, triacylglycerols, and lipid species containing different, mono- and polyunsaturated fatty acids, depending on the strain and growth condition. The strains also produced carbohydrates of industrial relevance. Chlorococcum sp. was found to be genetically transformable using standard simple transformation protocols. These results suggest that with further development, these strains could open the door to economic production of high value commercial compounds utilizing waste streams from cellulosic biomass.

经济地生产藻类生物质仍然是藻类产品商业化的主要瓶颈之一。这项工作的目的是鉴定可以在源自纤维素废物流的具有成本效益的培养基上生长的新藻类菌株，表征这些菌株生产高工业价值化合物的潜力，并鉴定那些能够轻松进行遗传转化的菌株。在这里，我们报告，在最初分离的 45 个菌株中，根据其以有机废料（玉米秸秆水解产物）作为碳源有效生长的能力，选择了 3 个菌株；绿球藻属 ，锁链藻属 sp。和衣藻。对每个菌株进行了非靶向代谢组学，鉴定了几种具有商业价值的相对丰度较高的代谢物，例如乳酸、2,3-丁二醇、氨基酸、酒石酸、三酰甘油和含有不同单脂肪酸的脂质种类。和多不饱和脂肪酸，取决于菌株和生长条件。这些菌株还产生具有工业相关性的碳水化合物。绿球藻属 被发现可以使用标准的简单转化方案进行遗传转化。这些结果表明，随着进一步发展，这些菌株可以为利用纤维素生物质废物流经济生产高价值商业化合物打开大门。