Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Fibronectin Connecting Cell Sheet Based on Click Chemistry for Wound Repair
Advanced Science ( IF 14.3 ) Pub Date : 2024-01-02 , DOI: 10.1002/advs.202306746 Wei Xu 1 , Meng He 1 , Qinghua Lu 1
Advanced Science ( IF 14.3 ) Pub Date : 2024-01-02 , DOI: 10.1002/advs.202306746 Wei Xu 1 , Meng He 1 , Qinghua Lu 1
Affiliation
|
As a living repair material, cell sheet exhibits significant potential in wound repair. Nonetheless, wound healing is a complicated and protracted process that necessitates specific repair functions at each stage, including hemostasis and antibacterial activity. In this work, on the basis of harvesting the cell sheet via a photothermal response strategy, a fibronectin attached cell sheet (FACS) is prepared to enhance its wound repair capability. For this purpose, the azide group (N3) is initially tagged onto the cell surface through metabolic glycoengineering of unnatural sugars, and then the conjugate (DBCO-fibronectin) comprises of the dibenzocyclooctyne (DBCO) and fibronectin with multiple wound repair functions is linked to N3 using click chemistry. Biological evaluations following this demonstrates that the FACS preparation exhibits excellent biocompatibility, and the fibronectin modification enhances the capacity for cell proliferation and migration. Moreover, in vivo wound healing experiment confirms the reparative efficacy of FACS. It not only has a wound closure rate 1.46 times that of a conventional cell sheet but also reduces inflammatory cell infiltration, promotes hair follicle and blood vessel regeneration, and encourages collagen deposition. This strategy holds enormous clinical potential and paves the way for advanced functional modifications of cell sheets.
中文翻译：
基于点击化学的纤连蛋白连接细胞片用于伤口修复
作为一种活体修复材料，细胞片在伤口修复方面表现出巨大的潜力。尽管如此，伤口愈合是一个复杂而漫长的过程，每个阶段都需要特定的修复功能，包括止血和抗菌活性。本工作在通过光热反应策略收获细胞片的基础上，制备了纤连蛋白附着细胞片（FACS）以增强其伤口修复能力。为此，首先通过非天然糖的代谢糖工程将叠氮基团（N 3 ）标记到细胞表面，然后将由二苯并环辛炔（DBCO）和具有多种伤口修复功能的纤连蛋白组成的缀合物（DBCO-纤连蛋白）连接起来使用点击化学合成N 3 。随后的生物学评价表明，FACS制剂表现出优异的生物相容性，纤连蛋白修饰增强了细胞增殖和迁移的能力。此外，体内伤口愈合实验证实了FACS的修复功效。它不仅伤口闭合率是传统细胞片的1.46倍，还能减少炎症细胞浸润，促进毛囊和血管再生，促进胶原蛋白沉积。该策略具有巨大的临床潜力，并为细胞片层的高级功能修饰铺平了道路。
更新日期：2024-01-02
中文翻译：
基于点击化学的纤连蛋白连接细胞片用于伤口修复
作为一种活体修复材料，细胞片在伤口修复方面表现出巨大的潜力。尽管如此，伤口愈合是一个复杂而漫长的过程，每个阶段都需要特定的修复功能，包括止血和抗菌活性。本工作在通过光热反应策略收获细胞片的基础上，制备了纤连蛋白附着细胞片（FACS）以增强其伤口修复能力。为此，首先通过非天然糖的代谢糖工程将叠氮基团（N 3 ）标记到细胞表面，然后将由二苯并环辛炔（DBCO）和具有多种伤口修复功能的纤连蛋白组成的缀合物（DBCO-纤连蛋白）连接起来使用点击化学合成N 3 。随后的生物学评价表明，FACS制剂表现出优异的生物相容性，纤连蛋白修饰增强了细胞增殖和迁移的能力。此外，体内伤口愈合实验证实了FACS的修复功效。它不仅伤口闭合率是传统细胞片的1.46倍，还能减少炎症细胞浸润，促进毛囊和血管再生，促进胶原蛋白沉积。该策略具有巨大的临床潜力，并为细胞片层的高级功能修饰铺平了道路。
京公网安备 11010802027423号