Simultaneous Identification of Major Thyroid Hormones by a Nickel Immobilized Biological Nanopore,Nano Letters

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Simultaneous Identification of Major Thyroid Hormones by a Nickel Immobilized Biological Nanopore
Nano Letters ( IF 9.6 ) Pub Date : 2023-12-27 , DOI: 10.1021/acs.nanolett.3c04024
Kefan Wang _{1,

2} , Xian Yang _{1,

2} , Yunqi Xiao _{1,

2} , Zhenyuan Cao _{1,

2} , Shanyu Zhang _{1,

2} , Panke Zhang ₁ , Shuo Huang _{1,

2}

Affiliation

Thyroid hormones (THs) are a variety of iodine-containing hormones that demonstrate critical physiological impacts on cellular activities. The assessment of thyroid function and the diagnosis of thyroid disorders require accurate measurement of TH levels. However, largely due to their structural similarities, the simultaneous discrimination of different THs is challenging. Nanopores, single-molecule sensors with a high resolution, are suitable for this task. In this paper, a hetero-octameric Mycobacterium smegmatis porin A (MspA) nanopore containing a single nickel ion immobilized to the pore constriction has enabled simultaneous identification of five representative THs including l-thyroxine (T4), 3,3′,5-triiodo-l-thyronine (T3), 3,3′,5′-triiodo-l-thyronine (rT3), 3,5-diiodo-l-thyronine (3,5-T2) and 3,3′-diiodo-l-thyronine (3,3′-T2). To automate event classification and avoid human bias, a machine learning algorithm was also developed, reporting an accuracy of 99.0%. This sensing strategy is also applied in the analysis of TH in a real human serum environment, suggesting its potential use in a clinical diagnosis.

中文翻译：

镍固定生物纳米孔同时鉴定主要甲状腺激素

甲状腺激素 (TH) 是多种含碘激素，对细胞活动具有重要的生理影响。甲状腺功能的评估和甲状腺疾病的诊断需要准确测量 TH 水平。然而，很大程度上由于它们的结构相似性，同时区分不同的 TH 具有挑战性。纳米孔，一种具有高分辨率的单分子传感器，适合这项任务。在本文中，含有固定在孔收缩处的单个镍离子的异八聚耻垢分枝杆菌孔蛋白 A (MspA) 纳米孔能够同时鉴定五种代表性 TH，包括l-甲状腺素 (T4)、3,3',5-三碘- l-甲状腺氨酸 (T3)、3,3′,5′-三碘-l-甲状腺氨酸 (rT3)、3,5-二碘-l-甲状腺氨酸 (3,5-T2) 和 3,3′-二碘-l -甲状腺氨酸（3,3'-T2）。为了实现事件分类自动化并避免人为偏差，还开发了机器学习算法，准确率高达 99.0%。这种传感策略也应用于真实人类血清环境中的 TH 分析，表明其在临床诊断中的潜在用途。

更新日期：2023-12-27

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