<br>对空气中禾本科（禾本科）花粉的环境 DNA 分析揭示了跨季节和气候带的分类多样性,Environmental Research

当前位置： X-MOL 学术 › Environ. Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

对空气中禾本科（禾本科）花粉的环境 DNA 分析揭示了跨季节和气候带的分类多样性
Environmental Research ( IF 7.7 ) Pub Date : 2023-12-30 , DOI: 10.1016/j.envres.2023.117983
Shanice Van Haeften ₁ , Bradley C Campbell ₁ , Andelija Milic ₁ , Elizabeth Addison-Smith ₁ , Jane Al Kouba ₂ , Alfredo Huete ₃ , Paul J Beggs ₂ , Janet M Davies ₁

Affiliation

草类遍布大多数生物地理区域，其多样性影响对花粉的过敏。此前，不同禾本科亚科对空气花粉的贡献主要是从历史植物标本室记录中推断出来的。我们最近在一个亚热带地点应用了环境 (e)DNA 元条形码，揭示了连续的空气传播草花粉峰源自 Chloridoid 和 Panicoid 草在一个季节内的重复开花。本研究旨在比较不同季节和气候区草花粉暴露的时空模式。使用常规体积撞击采样器并通过光学显微镜计数测定了 2017 年至 2019 年两个南方花粉季节亚热带（昆士兰州穆达皮利和罗克利）和温带（新南威尔士州麦格理公园和里士满）地点的空气花粉浓度。使用核糖体分类器，从每周收集一次的日常花粉样本中扩增出禾本科 rbcL 元条形码序列，并将其分配给亚科和属，并与澳大利亚生活地图集的目击记录进行比较。 eDNA 分析揭示了不同地点草花粉的不同优势模式：亚热带 Mutdapilly 和温带 Macquarie Park 地区盛行 Panicoid 草，而 Chloridoid 草则在亚热带 Rocklea 地区占主导地位。总体而言，亚热带地区显示出来自 Chloridoid 草的花粉比例显着高于温带地区，而温带地区显示出来自 Pooideae 草的花粉比例显着高于亚热带地区。 Pooid（春季）、Panicoid 和 Chloridoid（春末至秋季）以及 Arundinoid（秋季）花粉的空气传播时间与仲冬之后的天数显着相关。亚科的 eDNA 比例与气候带之间草目目击记录的分布相关。 eDNA 分析能够对跨季节和气候带的禾本科花粉记录进行更精细的分类识别，这对于了解草原对气候变化的适应以及过敏性呼吸道疾病患者花粉暴露的复杂性具有重要意义。

"点击查看英文标题和摘要"

Environmental DNA analysis of airborne poaceae (grass) pollen reveals taxonomic diversity across seasons and climate zones

Grasses populate most biogeographical zones, and their diversity influences allergic sensitisation to pollen. Previously, the contribution of different Poaceae subfamilies to airborne pollen has mostly been inferred from historical herbarium records. We recently applied environmental (e)DNA metabarcoding at one subtropical site revealing that successive airborne grass pollen peaks were derived from repeated flowering of Chloridoid and Panicoid grasses over a season. This study aimed to compare spatiotemporal patterns in grass pollen exposure across seasons and climate zones. Airborne pollen concentrations across two austral pollen seasons spanning 2017–2019 at subtropical (Mutdapilly and Rocklea, Queensland) and temperate (Macquarie Park and Richmond, New South Wales) sites, were determined with a routine volumetric impaction sampler and counting by light microscopy. Poaceae rbcL metabarcode sequences amplified from daily pollen samples collected once per week were assigned to subfamily and genus using a ribosomal classifier and compared with Atlas of Living Australia sighting records. eDNA analysis revealed distinct dominance patterns of grass pollen at various sites: Panicoid grasses prevailed in both subtropical Mutdapilly and temperate Macquarie Park, whilst Chloridoid grasses dominated the subtropical Rocklea site. Overall, subtropical sites showed significantly higher proportion of pollen from Chloridoid grasses than temperate sites, whereas the temperate sites showed a significantly higher proportion of pollen from Pooideae grasses than subtropical sites. Timing of airborne Pooid (spring), Panicoid and Chloridoid (late spring to autumn), and Arundinoid (autumn) pollen were significantly related to number of days from mid-winter. Proportions of eDNA for subfamilies correlated with distributions grass sighting records between climate zones. eDNA analysis enabled finer taxonomic discernment of Poaceae pollen records across seasons and climate zones with implications for understanding adaptation of grasslands to climate change, and the complexity of pollen exposure for patients with allergic respiratory diseases.

更新日期：2023-12-30

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南