Heteroatom doping and phase engineering are effective ways to promote the catalytic activity of nanoenzymes. Nitrogen-doped 1 T/2H mixed phase MoS₂/CuS heterostructure nanosheets N-1 T/2H-MoS₂/CuS are prepared by a simple hydrothermal approach using polyoxometalate (POM)-based metal–organic frameworks (MOFs) (NENU-5) as a precursor and urea as nitrogen doping reagent. The XPS spectroscopy (XPS) and Raman spectrum of N-1 T/2H-MoS₂/CuS prove the successful N-doping. NENU-5 was used as the template to prepare 1 T/2H-MoS₂/CuS with high content of 1 T phase by optimizing the reaction time. The use of urea as nitrogen dopant added to 1 T/2H-MoS₂/CuS, resulted in N-1 T/2H-MoS₂/CuS with an increase in the content of the 1 T phase from 80 % to 84 % and higher number of defects. N-1 T/2H-MoS₂/CuS shows higher peroxidase activity than 1 T/2H-MoS₂/CuS and a catalytic efficiency (K_cat/K_m) for H₂O₂ twice as high as that of 1 T/2H-MoS₂/CuS. The enhanced catalytic activity has probably been attributed to several reasons: (i) the insertion of urea during the hydrothermal process in the S-Mo-S layer of MoS₂, causing an increase in the interlayer spacing and in 1 T phase content, (ii) the replacement of S atoms in MoS₂ by N atoms from the urea decomposition, resulting in more defects and more active sites. As far as we know, N-1 T/2H-MoS₂/CuS nanosheets have the lowest detection limit (0.16 µm) for the colorimetric detection of hydroquinone among molybdenum disulfide-based catalysts. This study affords a new approach for the fabrication of high-performance nanoenzyme catalysts.

杂原子掺杂和相工程是提高纳米酶催化活性的有效途径。氮掺杂1T/2H混合相MoS ₂ /CuS异质结构纳米片N-1T/2H-MoS ₂ /CuS是使用多金属氧酸盐（POM）基金属有机框架（MOF）通过简单的水热方法制备的（NENU- 5)作为前驱体，尿素作为氮掺杂试剂。 N-1 T/2H-MoS ₂ /CuS的XPS光谱(XPS)和拉曼光谱证明N掺杂成功。以NENU-5为模板，通过优化反应时间，制备出高1T相含量的1T/2H-MoS ₂ /CuS 。将尿素作为氮掺杂剂添加到1T/2H-MoS ₂ /CuS中，得到N-1T/2H-MoS ₂ /CuS，其中1T相的含量从80%增加到84%，并且缺陷数量较多。 N-1 T/2H-MoS ₂ /CuS表现出比1 T/2H-MoS ₂ /CuS更高的过氧化物酶活性，并且对 H ₂ O ₂的催化效率（K _cat /K _m ）是1 T/的两倍。 2H-MoS ₂ /CuS 。 催化活性的增强可能归因于以下几个原因：（i）在水热过程中尿素插入MoS ₂的S-Mo-S层中，导致层间距和1 T相含量增加，（ ii) MoS ₂中的S原子被尿素分解中的N原子取代，导致更多的缺陷和更多的活性位点。据我们所知，在二硫化钼基催化剂中，N-1 T/2H-MoS ₂ /CuS纳米片对氢醌的比色检测具有最低的检测限（0.16 µm）。这项研究为制备高性能纳米酶催化剂提供了一种新方法。