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DNA Polymerase-Steered Self-Propelled and Self-Enhanced DNA Walker for Rapid and Distinctly Amplified Electrochemical Sensing
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-12-29 , DOI: 10.1021/acs.analchem.3c04340 Shufeng Liu 1 , Jialiang Wu 1 , Shuang Li 1 , Li Wang 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-12-29 , DOI: 10.1021/acs.analchem.3c04340 Shufeng Liu 1 , Jialiang Wu 1 , Shuang Li 1 , Li Wang 1
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The development of a simple, rapid, easy-to-operate, and ultrasensitive DNA walker-based sensing system is challenging but would be very intriguing for the enormous applications in biological analysis and disease monitoring. Herein, a new self-propelled and self-enhanced DNA walking strategy was developed on the basis of a simple DNA polymerase-steered conversion from a typical alternate DNA assembly process. The sensing platform was fabricated easily by immobilizing only one hairpin probe (H1) and the sensing process was based on a simple one-step mixing with another hairpin-like DNA probe (H2) and DNA polymerase. The DNA polymerization could achieve target recycling and successive DNA walking steps. Interestingly, along with each DNA walking step, the new DNA walker sequence could be autonomously accumulated for a self-enhanced DNA walking effect. This provided a multilevel signal amplification ability for the ultrasensitive detection of the target with a low detection limit of 0.18 fM. Moreover, it could greatly reduce the reaction time with the sensing process finished within 1 h. The detection selectivity and the applicative potential in a complicated biological matrix were also demonstrated. Furthermore, the flexible control of sensing modes (self-enhanced DNA walking or the alternate DNA assembly) by using DNA polymerase or not offered a powerful means for sensing performance modulation. It thus opens a new avenue toward the development of a DNA walker-based sensing platform with both rapid and ultrasensitive features and might hold a huge potential for point-of-care diagnostic applications.
中文翻译：
DNA 聚合酶引导的自驱动和自增强型 DNA Walker，可实现快速且明显放大的电化学传感
开发一种简单、快速、易于操作且超灵敏的基于 DNA 步行器的传感系统具有挑战性，但对于生物分析和疾病监测方面的巨大应用来说将是非常有趣的。在此，基于典型的替代 DNA 组装过程的简单 DNA 聚合酶引导转换，开发了一种新的自推进和自增强 DNA 行走策略。通过仅固定一个发夹探针 (H1) 即可轻松构建传感平台，并且传感过程基于与另一种发夹状 DNA 探针 (H2) 和 DNA 聚合酶的简单一步混合。 DNA聚合可以实现目标回收和连续的DNA行走步骤。有趣的是，随着每个DNA步行步骤，新的DNA步行者序列可以自主积累，以实现自我增强的DNA步行效果。这为目标的超灵敏检测提供了多级信号放大能力，检测限低至 0.18 fM。而且，它可以大大缩短反应时间，传感过程在1小时内完成。还证明了复杂生物基质中的检测选择性和应用潜力。此外，通过使用或不使用DNA聚合酶来灵活控制传感模式（自我增强的DNA行走或交替DNA组装）为传感性能调节提供了强大的手段。因此，它为开发基于 DNA walker 的传感平台开辟了一条新途径，该平台具有快速和超灵敏的特性，并且可能在护理点诊断应用中具有巨大的潜力。
更新日期：2023-12-29
中文翻译：
DNA 聚合酶引导的自驱动和自增强型 DNA Walker，可实现快速且明显放大的电化学传感
开发一种简单、快速、易于操作且超灵敏的基于 DNA 步行器的传感系统具有挑战性，但对于生物分析和疾病监测方面的巨大应用来说将是非常有趣的。在此，基于典型的替代 DNA 组装过程的简单 DNA 聚合酶引导转换，开发了一种新的自推进和自增强 DNA 行走策略。通过仅固定一个发夹探针 (H1) 即可轻松构建传感平台，并且传感过程基于与另一种发夹状 DNA 探针 (H2) 和 DNA 聚合酶的简单一步混合。 DNA聚合可以实现目标回收和连续的DNA行走步骤。有趣的是，随着每个DNA步行步骤，新的DNA步行者序列可以自主积累，以实现自我增强的DNA步行效果。这为目标的超灵敏检测提供了多级信号放大能力，检测限低至 0.18 fM。而且，它可以大大缩短反应时间，传感过程在1小时内完成。还证明了复杂生物基质中的检测选择性和应用潜力。此外，通过使用或不使用DNA聚合酶来灵活控制传感模式（自我增强的DNA行走或交替DNA组装）为传感性能调节提供了强大的手段。因此，它为开发基于 DNA walker 的传感平台开辟了一条新途径，该平台具有快速和超灵敏的特性，并且可能在护理点诊断应用中具有巨大的潜力。
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