<br>Muscone 通过 STAT3、MAPK 和 TGF-β/SMAD 信号通路抑制血管紧张素 II 诱导的心脏肥大,Molecular Biology Reports

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Muscone 通过 STAT3、MAPK 和 TGF-β/SMAD 信号通路抑制血管紧张素 II 诱导的心脏肥大
Molecular Biology Reports ( IF 2.6 ) Pub Date : 2023-12-29 , DOI: 10.1007/s11033-023-08916-1
Yi-Jiang Liu ₁ , Jia-Jia Xu ₁ , Cui Yang ₁ , Yan-Lin Li ₁ , Min-Wei Chen ₁ , Shi-Xiao Liu ₁ , Xiang-Hui Zheng _{1,

2} , Ping Luo ₁ , Rui Li ₁ , Di Xiao ₁ , Zhong-Gui Shan ₁

Affiliation

背景

麝香酮是从麝香中提取的化学单体。尽管许多研究已经证实了麝香酮的心脏保护作用，但麝香酮对心脏肥大的作用及其潜在机制尚不清楚。本研究的目的是探讨麝香酮对血管紧张素（Ang）II诱导的心脏肥大的作用。

方法和结果

在本研究中，我们首次发现麝香酮对Ang II诱导的小鼠心脏肥大和心脏损伤具有抑制作用。通过超声心动图测量分析心功能，并通过定量实时聚合酶链反应（qRT-PCR）、Masson三色染色和蛋白质印迹测定来确定心脏纤维化程度。其次，qRT-PCR实验表明，麝香酮通过减少促炎细胞因子的分泌和促进抗炎细胞因子的分泌来减轻心脏损伤。此外，蛋白质印迹分析发现，麝香酮通过抑制 STAT3、MAPK 和 TGF-β/SMAD 通路中关键蛋白的磷酸化来发挥心脏保护作用。另外，CCK-8和血清生化指标测定表明，麝香酮对正常细胞和器官无明显毒副作用。

结论

Muscone 可以部分通过抑制 STAT3、MAPK 和 TGF-β/SMAD 信号通路来减轻 Ang II 诱导的心脏肥大。

"点击查看英文标题和摘要"

Muscone inhibits angiotensin II–induced cardiac hypertrophy through the STAT3, MAPK and TGF-β/SMAD signaling pathways

Background

Muscone is a chemical monomer derived from musk. Although many studies have confirmed the cardioprotective effects of muscone, the effects of muscone on cardiac hypertrophy and its potential mechanisms are unclear.The aim of the present study was to investigate the effect of muscone on angiotensin (Ang) II-induced cardiac hypertrophy.

Methods and results

In the present study, we found for the first time that muscone exerted inhibitory effects on Ang II-induced cardiac hypertrophy and cardiac injury in mice. Cardiac function was analyzed by echocardiography measurement, and the degree of cardiac fibrosis was determined by the quantitative real-time polymerase chain reaction (qRT-PCR), Masson trichrome staining and western blot assay. Secondly, qRT-PCR experiment showed that muscone attenuated cardiac injury by reducing the secretion of pro-inflammatory cytokines and promoting the secretion of anti-inflammatory cytokines. Moreover, western blot analysis found that muscone exerted cardio-protective effects by inhibiting phosphorylation of key proteins in the STAT3, MAPK and TGF-β/SMAD pathways. In addition, CCK-8 and determination of serum biochemical indexes showed that no significant toxicity or side effects of muscone on normal cells and organs.