<br>全氟辛烷磺酸类物质对斑马鱼幼虫结构相关的甲状腺破坏作用,Environmental Science & Technology

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全氟辛烷磺酸类物质对斑马鱼幼虫结构相关的甲状腺破坏作用
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2023-12-29 , DOI: 10.1021/acs.est.3c07003
Shujun Yi ₁ , Jingwen Wang ₁ , Rouyi Wang ₁ , Menglin Liu ₁ , Wenjue Zhong ₁ , Lingyan Zhu ₁ , Guibin Jiang ₂

Affiliation

氯化多氟辛烷醚磺酸盐 (6:2 Cl-PFESA)、氢化多氟辛烷醚磺酸盐 (6:2 H-PFESA) 和氯化多氟辛烷磺酸盐 (Cl-PFOS) 与受管制的全氟辛烷磺酸盐 (PFOS) 结构相似，但其潜在毒性为很少有人知道。在这里，对这四种化合物对斑马鱼幼虫的甲状腺破坏潜力进行了比较研究。 PFOS、Cl-PFOS 和 6:2 Cl-PFESA 在幼虫中的积累水平相似，大约比 6:2 H-PFESA 高 1.3-1.6 倍。此外，PFOS、Cl-PFOS 和 6:2 Cl-PFESA 在基因调控方面表现出比 6:2 H-PFESA 更强的破坏，特别是在甲状腺激素 (TH) 激活和作用以及游离形式和总形式的 TH 稳态方面。甲状腺素 (T4) 和 3,5,3'-三碘甲状腺原氨酸 (T3)。这些结果表明，氯化或氧插入不会显着改变 PFOS 的甲状腺毒性，但氢化会减轻它。分子对接分析和荧光素酶报告基因测定提供了机制观点，即全氟辛烷磺酸类物质可以竞争性地取代 TH 与 TH 血浆和膜转运蛋白结合，从而分别破坏 TH 转运和作用。此外，它们还能够通过^I的 Na ⁺ /K ⁺依赖性转运或与脱碘酶位点的竞争性结合来破坏 TH 合成和激活。

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Structure-Related Thyroid Disrupting Effect of Perfluorooctanesulfonate-like Substances in Zebrafish Larvae

Chlorinated polyfluorooctane ether sulfonate (6:2 Cl-PFESA), hydrogenated polyfluorooctane ether sulfonate (6:2 H-PFESA), and chlorinated polyfluorooctanesulfonate (Cl-PFOS) share structural similarities with the regulated perfluorooctanesulfonate (PFOS), but their toxic potential is rarely known. Here, the thyroid disrupting potential of these four compounds in zebrafish larvae has been comparably investigated. PFOS, Cl-PFOS, and 6:2 Cl-PFESA were accumulated in the larvae at similar levels, approximately 1.3–1.6 times higher than 6:2 H-PFESA. Additionally, PFOS, Cl-PFOS, and 6:2 Cl-PFESA exhibited stronger disruption than 6:2 H-PFESA on genetic regulation, particularly concerning thyroid hormone (TH) activation and action and on TH homeostasis in both free and total forms of thyroxine (T4) and 3,5,3′-triiodothyronine (T3). These results indicate that chlorination or oxygen insertion does not substantially alter the thyrotoxicity of PFOS, but hydrogenation mitigates it. Molecular docking analysis and the luciferase reporter gene assay provided mechanistic perspectives that the PFOS-like substances could competitively replace THs to bind with TH plasma and membrane transporters, thereby disrupting TH transport and action, respectively. Moreover, they are also potent to disrupt TH synthesis and activation through Na⁺/K⁺-dependent transport of I^– or competitive binding to the sites of deiodinases.

更新日期：2023-12-29

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