This work investigated the impact of a waste-derived carbon source, crude glycerol (CG), on Anammox. Batch bioassays were conducted to identify inhibitory component(s) in CG, and the relationship between Anammox activity and the concentration of CG, pure glycerol, and methanol were assessed. The results showed that the half-maximal inhibitory concentration of CG and methanol are 434.5 ± 51.8 and 143.0 ± 19.6 mg chemical oxygen demand (COD) L^-1, respectively, while pure glycerol at 0–2283 mg COD L^-1 had no significant adverse effect on Anammox. The results suggested methanol is the major inhibitor in CG via a non-competitive inhibition mechanism. COD/total inorganic nitrogen ratio of > 1.3 was observed to cause a significant Anammox inhibition (>20 %), especially at low substrate level. These results are valuable for evaluating the feasibility of using CG for nitrogen removal in water resource recovery facilities, promoting sustainable development.

这项工作研究了废物来源的碳源粗甘油 (CG) 对厌氧氨氧化的影响。通过批量生物测定来鉴定 CG 中的抑制成分，并评估 Anammox 活性与 CG、纯甘油和甲醇浓度之间的关系。结果表明，CG和甲醇的半最大抑制浓度分别为434.5±51.8和143.0±19.6 mg化学需氧量(COD) L ^-1 ，而纯甘油在0-2283 mg COD L ^-1时没有显着的抑制作用。对厌氧氨氧化产生不利影响。结果表明，甲醇通过非竞争性抑制机制成为 CG 的主要抑制剂。据观察，COD/总无机氮比率 > 1.3 会导致显着的厌氧氨氧化抑制 (> 20 %)，特别是在底物水平较低的情况下。这些结果对于评估在水资源回收设施中使用CG脱氮的可行性，促进可持续发展具有重要价值。