Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) (B. dorsalis) is an important agricultural, major invasive, and quarantine pest that can cause significant damage to the economic value of the fruit and vegetable industry. Male bait is one of the most effective methods of surveying, monitoring, and controlling B. dorsalis. In our study, we constructed cDNA libraries using total RNA extracted independently from the antennae, mouthparts, and thoracic legs of male and female adults and the ovipositors of female adults and screened out four aldehyde-oxidase-related genes (AOX-related), C58800, C66700, C67485, and C67698. Molecular docking predictions showed that eight compounds, including 3,4-dimethoxycinnamyl alcohol, 3,4-dimethoxy-cinnamaldehyde, deet, ethyl N-acetyl-N-butyl-β-alaninate, n-butyl butyrate, n-butyl butyrate, ethyl butyrate, methyl eugenol, and ethyl acetate, could combine with proteins encoded by the four B. dorsalis AOX-related genes. Furthermore, QPCR was performed to confirm that four compounds, including 3,4-dimethoxy cinnamic aldehyde, butyl levulinic acid ethyl ester (mosquito repellent), butyl butyrate, and methyl eugenol, induced significant changes in the AOX-related genes of B. dorsalis. These results provide useful information and guidance for the batch screening of potentially useful compounds and the search for effective attractants of B. dorsalis.

中文翻译：

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9 种化合物对 Bactrocera dorsalis （Hendel） 醛氧化酶相关基因的影响


Bactrocera dorsalis （Hendel） （双翅目：Tephritidae） （B. dorsalis） 是一种重要的农业、主要入侵和检疫性害虫，可对果蔬行业的经济价值造成重大损害。雄性诱饵是调查、监测和控制背饵 B. doral 的最有效方法之一。在我们的研究中，我们使用独立于雄性和雌性成虫的触角、口器和胸肢以及雌性成虫的产卵器提取的总 RNA 构建了 cDNA 文库，并筛选出 4 个醛氧化酶相关基因 （AOX 相关） C58800 、 C66700 、 C67485 和 C67698。分子对接预测显示，3,4-二甲氧基肉桂醇、3,4-二甲氧基肉桂醛、避蚊胺、N-乙酰基-N-丁基-β-丙氨酸乙酯、丁酸正丁酯、丁酸正丁酯、丁酸乙酯、丁香酚甲酯和乙酸乙酯等 8 种化合物可以与 4 个背双歧杆菌 AOX 相关基因编码的蛋白质结合。此外，进行 QPCR 以确认 3,4-二甲氧基肉桂醛、丁基乙酰丙酸乙酯（驱蚊剂）、丁酸丁酯和丁香酚甲基丁香酚 4 种化合物诱导了背双歧杆菌 AOX 相关基因的显着变化。这些结果为批量筛选可能有用的化合物和寻找背侧双歧杆菌的有效引诱剂提供了有用的信息和指导。