A novel negative-stranded RNA mycovirus was isolated from the phytopathogenic fungus Fusarium sibiricum strain AH32. This virus, tentatively named "Fusarium sibiricum coguvirus 1" (FsCV1), has a bipartite genome consisting of two RNA segments (RNA1 and RNA2). The negative-sense RNA1 is 6711 nt in length, encoding the RNA-dependent RNA polymerase (RdRp, p251) in the viral complementary (vc) strand. The ambisense RNA2 (1204 nt long) encodes two proteins from overlapping genes: the nucleocapsid protein (NP, p38) in the vc strand and a protein of unknown function (UFP, p36) in the viral (v) strand. In contrast to other Bunyavirales members, in FsCV1, the two open reading frames are separated by a long AU-rich intergenic region (IR). Sequence comparisons and phylogenetic analysis based on RdRp and NP sequences demonstrated that FsCV1 is a novel bipartite negative-stranded RNA mycovirus of the genus Coguvirus, family Phenuiviridae, order Bunyavirales.

从植物病原真菌西伯利亚镰刀菌菌株 AH32 中分离出一种新型负链 RNA 真菌病毒。该病毒暂定名为“西伯利亚镰刀菌病毒 1 号”(FsCV1)，具有由两个 RNA 片段（RNA1 和 RNA2）组成的二分基因组。负义 RNA1 长度为 6711 nt，编码病毒互补 (vc) 链中的 RNA 依赖性 RNA 聚合酶 (RdRp、p251)。双义RNA2（1204 nt长）编码来自重叠基因的两种蛋白质：vc链中的核衣壳蛋白（NP，p38）和病毒（v）链中的未知功能蛋白（UFP，p36）。与其他布尼亚病毒目成员相比，在 FsCV1 中，两个开放阅读框被一个长的富含 AU 的基因间区域 (IR) 分开。基于RdRp和NP序列的序列比较和系统发育分析表明， FsCV1是布尼亚病毒目苯鸟病毒科Coguvirus属的一种新型二分负链RNA真菌病毒。