当前位置：
X-MOL 学术
›
Chem. Biodivers.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Phosphorylation Modification, Structural Characterization, Antioxidant and DNA Protection Capacities of Polysaccharides from Asarum Sieboldii Miq
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2023-12-26 , DOI: 10.1002/cbdv.202301781 Shun-He Yang 1 , Xiao-Li Wang 2 , Hao-Nan Zhang 1 , Li-Fei Zhu 2 , Shu-Hao Qu 2 , Ming-Yue Zhang 1 , Hong Zhang 1 , Peng-Fei Liu 1
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2023-12-26 , DOI: 10.1002/cbdv.202301781 Shun-He Yang 1 , Xiao-Li Wang 2 , Hao-Nan Zhang 1 , Li-Fei Zhu 2 , Shu-Hao Qu 2 , Ming-Yue Zhang 1 , Hong Zhang 1 , Peng-Fei Liu 1
Affiliation
|
Polysaccharide from Asarum sieboldii Miq (ASP) was extracted and five phosphorylation polysaccharides with different degree of substitution were obtained, namely ASPP1, ASPP2, ASPP3, ASPP4, and ASPP5 (ASPPs). The physical and chemical structure and biological activities were studied. The results suggested that the carbohydrate and protein content were reduced while uronic acid was increased after phosphorylation modification. The molecular weight of ASPPs was significantly lower than that of ASP. ASPPs were acidic heteropolysaccharides mainly composed of galacturonic acid, galactose, glucose, fructose, and arabinose. The UV-vis spectrum indicated that the polysaccharides did not contain nucleic acid or protein after modification. The Fourier transform infrared spectrum demonstrated that ASPPs contained characteristic absorption peaks of P=O and P−O-C near 1270 and 980 cm−1. ASPPs presented a triple helix conformation, but it was not presented in ASP. The scanning electron microscopy analysis showed that the surface topography and particle structure of ASP were different after modification. Compared with ASP, ASPPs enhanced the activity to scavenge DPPH and ABTS free radicals and possessed more protective ability to DNA oxidation caused by OH⋅, GS⋅, and AAPH free radicals. These results suggest that chemical modification is beneficial for the exploitation and utilization of natural polysaccharides.
中文翻译：
细辛多糖的磷酸化修饰、结构表征、抗氧化和 DNA 保护能力
提取细辛多糖(ASP)，得到5种不同取代度的磷酸化多糖，即ASPP1、ASPP2、ASPP3、ASPP4和ASPP5(ASPPs)。研究了其理化结构和生物活性。结果表明，磷酸化修饰后，碳水化合物和蛋白质含量降低，糖醛酸含量增加。 ASPPs的分子量明显低于ASP。 ASPPs是一种酸性杂多糖，主要由半乳糖醛酸、半乳糖、葡萄糖、果糖和阿拉伯糖组成。紫外可见光谱表明修饰后的多糖不含核酸和蛋白质。傅里叶变换红外光谱表明ASPPs在1270和980 cm -1附近含有P=O和P−OC的特征吸收峰。 ASPP 呈现三螺旋构象，但 ASP 中没有呈现。扫描电镜分析表明改性后三元复合材料的表面形貌和颗粒结构发生了变化。与ASP相比，ASPPs增强了清除DPPH和ABTS自由基的活性，对OH·、GS·和AAPH自由基引起的DNA氧化具有更强的保护能力。这些结果表明化学修饰有利于天然多糖的开发和利用。
更新日期：2023-12-26
中文翻译：
细辛多糖的磷酸化修饰、结构表征、抗氧化和 DNA 保护能力
提取细辛多糖(ASP)，得到5种不同取代度的磷酸化多糖，即ASPP1、ASPP2、ASPP3、ASPP4和ASPP5(ASPPs)。研究了其理化结构和生物活性。结果表明，磷酸化修饰后，碳水化合物和蛋白质含量降低，糖醛酸含量增加。 ASPPs的分子量明显低于ASP。 ASPPs是一种酸性杂多糖，主要由半乳糖醛酸、半乳糖、葡萄糖、果糖和阿拉伯糖组成。紫外可见光谱表明修饰后的多糖不含核酸和蛋白质。傅里叶变换红外光谱表明ASPPs在1270和980 cm -1附近含有P=O和P−OC的特征吸收峰。 ASPP 呈现三螺旋构象，但 ASP 中没有呈现。扫描电镜分析表明改性后三元复合材料的表面形貌和颗粒结构发生了变化。与ASP相比，ASPPs增强了清除DPPH和ABTS自由基的活性，对OH·、GS·和AAPH自由基引起的DNA氧化具有更强的保护能力。这些结果表明化学修饰有利于天然多糖的开发和利用。
京公网安备 11010802027423号