The liver plays a crucial role in several important processes in the human body, including metabolism, detoxification, and immune function. When the liver experiences acute injury, it can cause significant harm and requires prompt detection. Traditional biomarkers lack specificity and cannot detect changes in real-time, making them unsuitable for monitoring pathological processes. Recent studies have shown that acute liver injury (ALI) is closely related to oxidative stress, with peroxynitrite (ONOO⁻) being a vital byproduct of liver metabolism and become a critical biomarker for detecting liver damage. As a result, this research developed an activatable near-infrared fluorescent probe W-3a that can be used to detect endogenous ONOO⁻ in a mouse model of ALI induced by lipopolysaccharides (LPS). The probe has high selectivity and anti-interference ability, with a reaction time <10 min and a detection limit of 85 nM. It was successfully utilized in detecting endogenous ONOO⁻ in cells and live imaging of ALI mice.

肝脏在人体的几个重要过程中发挥着至关重要的作用，包括新陈代谢、解毒和免疫功能。当肝脏遭受急性损伤时，会造成重大伤害，需要及时发现。传统的生物标志物缺乏特异性，无法实时检测变化，不适合监测病理过程。最近的研究表明，急性肝损伤（ALI）与氧化应激密切相关，过氧亚硝酸盐（ONOO ^- ）是肝脏代谢的重要副产物，成为检测肝损伤的关键生物标志物。因此，本研究开发了一种可激活的近红外荧光探针 W-3a，可用于检测脂多糖 (LPS) 诱导的 ALI 小鼠模型中的内源性^ONOO 。该探针具有高选择性和抗干扰能力，反应时间<10 min，检测限为85 nM。它已成功用于检测细胞中的内源性 ONOO ^-以及 ALI 小鼠的活体成像。