Mobilized Electrospray Device for On-Tissue Chemical Derivatization in MALDI-MS Imaging,Journal of the American Society for Mass Spectrometry

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Mobilized Electrospray Device for On-Tissue Chemical Derivatization in MALDI-MS Imaging
Journal of the American Society for Mass Spectrometry ( IF 3.1 ) Pub Date : 2023-12-26 , DOI: 10.1021/jasms.3c00290
Andrew E Paulson ₁ , Evan A Larson ₁ , Young Jin Lee ₁

Affiliation

Applying solutions of a matrix or derivatization agent via microdroplets is a common sample preparation technique for matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) experiments. Mobilized nebulizer sprayers are commonly used to create a homogeneous matrix or reagent layer across large surfaces. Electrospray devices have also been used to produce microdroplets for the same purpose but are rarely used for large tissues due to their immobility. Herein, we present a movable electrospray device that can be used for large tissue sample preparation through a simple modification to an automatic commercial nebulizer device. As demonstrated for on-tissue chemical derivatization (OTCD) with Girard’s reagent T using a mimetic tissue model, the sprayer has the additional benefit of being able to investigate reaction acceleration in OTCD when comparing electrostatically charged spray to electrostatically neutral spray. Finally, MALDI-MSI of fatty aldehydes is successfully demonstrated in rat brain tissues using this device for both OTCD and matrix application.

中文翻译：

用于 MALDI-MS 成像中组织化学衍生化的移动式电喷雾装置

通过微滴应用基质或衍生剂溶液是基质辅助激光解吸/电离质谱成像 (MALDI-MSI) 实验的常见样品制备技术。移动式雾化器通常用于在大表面上形成均匀的基质或试剂层。电喷雾装置也已用于产生用于相同目的的微滴，但由于其固定性而很少用于大型组织。在此，我们提出了一种可移动电喷雾装置，通过对自动商用雾化器装置进行简单修改，可用于大组织样品制备。正如使用模拟组织模型使用 Girard 试剂 T 进行的组织化学衍生化 (OTCD) 所证明的那样，喷雾器的另一个优点是在比较带静电喷雾与静电中性喷雾时能够研究 OTCD 中的反应加速。最后，使用该装置在 OTCD 和基质应用中成功地在大鼠脑组织中证明了脂肪醛的 MALDI-MSI。

更新日期：2023-12-26

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