Developing antibacterial collagen-based wound dressings that target multidrug-resistant bacteria holds promise for healing diabetic wounds prone to infection, yet traditional approaches pose persistent challenges of cytotoxicity and uneconomical steps. To address these issues, we develop a biocompatible, eco-friendly, one-step cross-linking and reduction strategy, fabricating injectable antibacterial collagen hydrogels containing in situ-generated silver nanoparticles for diabetic wound care. By exposing the collagen-silver ion (Ag+) mixture to visible light, collagen-silver nanoparticle (Col I/AgNPs) hydrogels were one step created, eliminating the need for additional cross-linkers and toxic reducing agents. The synergistic interaction between collagen and silver ions was the first time investigated, revealing a pivotal redox reaction with a dual role that simultaneously initiated collagen cross-linking and reduced Ag+ to AgNPs. The resulting biocompatible Col I/AgNPs hydrogel was found to have multifunctional properties, exhibiting excellent injectability, enhanced biological activity, and outstanding broad-spectrum antimicrobial ability. Furthermore, animal experiments utilizing a diabetic full-thickness wound model demonstrated their excellent in vivo healing efficacies, capable of establishing efficient antibacterial barriers and serving as bioactive regenerative scaffolds. Our study proposes an innovative one-step approach to create injectable antibacterial collagen-silver nanoparticle hydrogels, highlighting their potential as advanced dressings for diabetic wound treatment and other biomedical applications.

中文翻译：

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原位合成银纳米颗粒一步制备可注射抗菌胶原水凝胶，加速糖尿病伤口愈合


开发针对多重耐药细菌的抗菌胶原蛋白伤口敷料有望治愈容易感染的糖尿病伤口，但传统方法面临细胞毒性和不经济步骤的持续挑战。为了解决这些问题，我们开发了一种生物相容性、环保、一步交联和还原策略，制造含有原位生成的银纳米粒子的可注射抗菌胶原蛋白水凝胶，用于糖尿病伤口护理。通过将胶原蛋白-银离子 (Ag+) 混合物暴露在可见光下，一步生成了胶原蛋白-银纳米颗粒 (Col I/AgNPs) 水凝胶，无需额外的交联剂和有毒还原剂。首次研究了胶原蛋白和银离子之间的协同相互作用，揭示了具有双重作用的关键氧化还原反应，可同时引发胶原蛋白交联和将 Ag+ 还原为 AgNP。由此产生的生物相容性 Col I/AgNPs 水凝胶具有多功能特性，表现出优异的可注射性、增强的生物活性和出色的广谱抗菌能力。此外，利用糖尿病全层伤口模型的动物实验证明了它们优异的体内愈合功效，能够建立有效的抗菌屏障并充当生物活性再生支架。我们的研究提出了一种创新的一步法来制造可注射的抗菌胶原银纳米颗粒水凝胶，突显了它们作为糖尿病伤口治疗和其他生物医学应用的先进敷料的潜力。