蛋白质组学和代谢组学分析阐明 PEDV 对约克夏仔猪的影响并揭示 PEDV 反应的潜在分子机制,Agriculture

当前位置： X-MOL 学术 › Agriculture › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

蛋白质组学和代谢组学分析阐明 PEDV 对约克夏仔猪的影响并揭示 PEDV 反应的潜在分子机制
Agriculture ( IF 2.072 ) Pub Date : 2023-12-26 , DOI: 10.3390/agriculture14010043
Lijun Shi ₁ , Huihui Li ₁ , Chunxiang Zhou ₂ , Lixian Wang ₁

Affiliation

猪流行性腹泻病毒（PEDV）是一种RNA病毒，可引起仔猪急性腹泻、呕吐、脱水和高死亡率，给全球养猪业带来重大挑战。然而，仔猪 PEDV 的分子机制尚未得到很好的理解。在本研究中，我们构建了约克夏仔猪模型（对照组、耐药组和易感组）来进行空肠蛋白质组学和血清代谢组学研究。总共鉴定出734个差异表达蛋白（DEP）和208个显着差异累积代谢物（DAM），功能注释显示它们主要参与代谢和信号转导途径。此外，我们使用加权基因共表达网络分析（WGCNA）、短时间序列表达挖掘器（STEM）和随机森林分析来检测每个相应组的关键有希望的生物标志物。通过构建蛋白质和代谢物之间的关系，我们挖掘了生物标志物并解释了它们对PEDV反应的分子机制。这些数据和结果可能是 PEDV 感染的良好资源，并为分子 PEDV 反应机制提供了宝贵的见解。

"点击查看英文标题和摘要"

Proteomic and Metabolomic Profiling Elucidate the Impact of PEDV on Yorkshire Piglets and Reveal the Underlying Molecular Mechanism of PEDV Response

Porcine epidemic diarrhea virus (PEDV) is an RNA virus causing acute diarrhea, vomiting, dehydration and high mortality in piglets, and poses significant challenges for the global pig industry. However, the molecular mechanism underlying PEDV in piglets has not been well comprehended. In this study, we constructed the Yorkshire piglet model (control, resistance, and susceptible groups) to perform the proteomics of jejunum and metabolomics of serum. In total, 734 differentially expressed proteins (DEPs) and 208 significant differentially accumulated metabolites (DAMs) were identified, and the functional annotation showed that they were mainly involved in metabolic and signal transduction pathways. Further, we used weighted gene co-expression network analysis (WGCNA), short timeseries expression miner (STEM) and random forest analyses to detect key promising biomarkers for each corresponding group. By constructing the relationships between proteins and metabolites, we excavated the biomarkers and explained their molecular mechanism of the PEDV response. These data and results might be good resources for the PEDV infection and offer valuable insights into the molecular PEDV response mechanisms.

更新日期：2023-12-26

点击分享查看原文

点击收藏

公开下载