Brochothrix thermosphacta is considered as a major food spoiler bacteria. This study evaluates biofilm formation by B. thermosphacta CD337(2) – a strong biofilm producer strain – on three food industry materials (polycarbonate (PC), polystyrene (PS), and stainless steel (SS)). Biofilms were continuously grown under flow at 25 °C in BHI broth in a modified CDC biofilm reactor. Bacterial cells were enumerated by plate counting, and biofilm spatial organization was deciphered by combining confocal laser scanning microscopy and image analysis. The biofilms had the same growth kinetics on all three materials and reach 8log CFU/cm² as maximal concentration. Highly structured biofilms were observed on PC and PS, but less structured ones on SS. This difference was confirmed by structural quantification analysis using the image analysis software tool BiofilmQ. Biofilm on SS show less roughness, density, thickness and volume. The biofilm 3D structure seemed to be related to the coupon topography and roughness.

The materials used in this study do not affect biofilm growth. However, their roughness and topography affect the biofilm architecture, which could influence biofilm behaviour.

Brochothrix thermosphacta被认为是主要的食物腐败细菌。本研究评估了热环芽孢杆菌CD337(2)（一种强生物膜生产菌株）在三种食品工业材料（聚碳酸酯 (PC)、聚苯乙烯 (PS) 和不锈钢 (SS)）上形成的生物膜。在改进的 CDC 生物膜反应器中，生物膜在 BHI 肉汤中于 25 °C 流动下连续生长。通过平板计数对细菌细胞进行计数，并通过结合共焦激光扫描显微镜和图像分析来解读生物膜的空间组织。生物膜在所有三种材料上具有相同的生长动力学，并且最大浓度达到8log CFU/cm ² 。在 PC 和 PS 上观察到高度结构化的生物膜，但在 SS 上观察到结构化程度较低的生物膜。使用图像分析软件工具 BiofilmQ 进行结构定量分析证实了这种差异。 SS 上的生物膜表现出较小的粗糙度、密度、厚度和体积。生物膜 3D 结构似乎与优惠券的形貌和粗糙度有关。

本研究中使用的材料不影响生物膜的生长。然而，它们的粗糙度和形貌会影响生物膜的结构，从而影响生物膜的行为。