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Process integration for efficient conversion of cassava peel waste into polyhydroxyalkanoates
Journal of Environmental Chemical Engineering ( IF 7.4 ) Pub Date : 2023-12-25 , DOI: 10.1016/j.jece.2023.111815 Carmen Hierro-Iglesias , Cornelius O. Fatokun , Annie Chimphango , Richard Bayitse , Paula Helena Blanco-Sanchez , Patricia Thornley , Alfred Fernandez-Castane
Journal of Environmental Chemical Engineering ( IF 7.4 ) Pub Date : 2023-12-25 , DOI: 10.1016/j.jece.2023.111815 Carmen Hierro-Iglesias , Cornelius O. Fatokun , Annie Chimphango , Richard Bayitse , Paula Helena Blanco-Sanchez , Patricia Thornley , Alfred Fernandez-Castane
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Polyhydroxyalkanoates (PHA) are biodegradable polyesters that can be produced from renewable resources. However, PHA biomanufacturing is costly compared to petrochemical-based plastics. A promising solution consists of using cassava () waste, abundant biomass in developing countries, as a carbon source for PHA production. This study involved characterising untreated and acid-hydrolysed cassava peel (CP) to confirm the degradation of polysaccharides into fermentable sugars after pre-treatment. A chemical and biological integrated process was developed, optimising the pre-treatment using a central composite design. The highest conversion of CP into reducing sugars was 97% (w/w) using 3 M HSO, 120 min and 90 ºC. The ability of to grow on CP hydrolysate and produce PHA was screened resulting in up to OD 15.8 and 1.5 g/L of PHA (31% (g/g)). Flow cytometry allowed rapid, simple, and high-throughput assessment of PHA content. These findings pave the way for developing a biorefinery platform for PHA production from cassava waste.
更新日期：2023-12-25
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