A novel method for measuring specific anammox activity (SAA) was proposed based on continuous flow tests to accurately determine the SAA of anammox sludge from continuous flow reactors, resolving the challenges of inaccurate SAA assessment caused by substrate shock to anammox bacteria. Results showed SAA of expanded granular sludge bed sludge via batch tests (0.101 ± 0.018 g-N·g-VSS^-1·d^-1) was lower than continuous flow tests (0.206 ± 0.010 g-N·g-VSS^-1·d^-1) (p < 0.05), highlighting the impact of substrate shock. Conversely, SAA of sequencing batch reactor sludge assessed via batch tests (0.878 ± 0.008 g-N·g-VSS^-1·d^-1) was higher than continuous flow tests (0.809 ± 0.005 g-N·g-VSS^-1·d^-1) (p < 0.01), attributed to endogenous denitrification. The advantages of continuous flow tests over batch tests included milder feeding way, stricter anaerobic conditions, and minimal sampling impact on system. Our study contributes to more accurate measurements of SAA of anammox sludge from continuous flow reactors, favoring long-term robust operation of anammox reactors.

提出了一种基于连续流测试的厌氧氨氧化比活度（SAA）测量新方法，以准确测定连续流反应器中厌氧氨氧化污泥的SAA，解决了厌氧氨氧化菌底物冲击造成的SAA评估不准确的难题。结果表明，批量试验的膨胀颗粒污泥床污泥的SAA（0.101±0.018 gN·g-VSS ^-1 ·d ^-1 ）低于连续流动试验（0.206±0.010 gN·g-VSS ^-1 ·d ^-1 ） ( p < 0.05)，突出了基材冲击的影响。相反，通过间歇测试评估的序批式反应器污泥的SAA (0.878 ± 0.008 gN·g-VSS ^-1 ·d ^-1 ) 高于连续流动测试(0.809 ± 0.005 gN·g-VSS ^-1 ·d ^-1 ) ( p < 0.01)，归因于内源反硝化。连续流测试相对于批量测试的优点包括更温和的进料方式、更严格的厌氧条件以及对系统的最小采样影响。我们的研究有助于更准确地测量连续流反应器中的厌氧氨氧化污泥的SAA，有利于厌氧氨氧化反应器的长期稳健运行。