The glucose transporter 1 (GLUT1) protein is involved in the basal-level absorption of glucose in tumor cells. Inhibiting GLUT1 decreases tumor cell proliferation and induces tumor cell damage. Natural GLUT1 inhibitors have been studied only to a small extent, and the structures of known natural GLUT1 inhibitors are limited to a few classes of natural products. Therefore, discovering and researching other natural GLUT1 inhibitors with novel scaffolds are essential. Physalis angulata L. var. villosa is a plant known as Mao-Ku-Zhi (MKZ). Withanolides are the main phytochemical components of MKZ. MKZ extracts and the components of MKZ exhibited antitumor activity in recent pharmacological studies. However, the antitumor-active components of MKZ and their molecular mechanisms remain unknown. A cell membrane-biomimetic nanoplatform (CM@Fe₃O₄/MIL-101) was used for target separation of potential GLUT1 inhibitors from MKZ. A new withanolide, physagulide Y (2), together with six known withanolides (1, 3–7), was identified as a potential GLUT1 inhibitor. Physagulide Y was the most potent GLUT1 inhibitor, and its antitumor activity and possible mechanism of action were explored in MCF-7 human cancer cells. These findings advance the development of technologies for the targeted separation of natural products and identify a new molecular framework for the investigation of natural GLUT1 inhibitors.

中文翻译：

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酸浆中基于茄内酯的葡萄糖转运蛋白 1 抑制剂的靶标分离和潜在抗癌活性绒毛


葡萄糖转运蛋白 1 (GLUT1) 蛋白参与肿瘤细胞中葡萄糖的基础水平吸收。抑制 GLUT1 可减少肿瘤细胞增殖并诱导肿瘤细胞损伤。天然 GLUT1 抑制剂的研究范围很小，已知的天然 GLUT1 抑制剂的结构仅限于几类天然产物。因此，发现和研究其他具有新型支架的天然GLUT1抑制剂至关重要。酸浆 (Physalis angulata L. var.)绒毛是一种被称为毛苦枝（MKZ）的植物。醉茄内酯是 MKZ 的主要植物化学成分。 MKZ 提取物和 MKZ 成分在最近的药理学研究中表现出抗肿瘤活性。然而，MKZ 的抗肿瘤活性成分及其分子机制仍不清楚。细胞膜仿生纳米平台（CM@Fe ₃ O ₄ /MIL-101）用于从MKZ中分离潜在的GLUT1抑制剂。一种新的睡茄内酯 physagulide Y ( 2 ) 以及六种已知的睡茄内酯 ( 1 , 3 – 7 ) 被确定为潜在的 GLUT1 抑制剂。 Physagulide Y 是最有效的 GLUT1 抑制剂，其抗肿瘤活性和可能的​​作用机制在 MCF-7 人类癌细胞中进行了探索。这些发现推动了天然产物靶向分离技术的发展，并确定了用于研究天然 GLUT1 抑制剂的新分子框架。