Substantial efforts are underway to tackle the current challenges of sustainability and environmental impacts linked to orthodox animal agriculture. This had led to advancement in food innovation guiding the fabrication of edible scaffolds based cultured meat. This current research work aims to develop and validate a new approach in fabricating a 3D porous scaffold of decellularized apple coated with a polymer mixture of gelatin/alginate for cultivated meat production. The fabricated noncoated (A) and coated (CA) 3D scaffolds presented different ratios of pore sizes with the medium-sized pores (100–250 µm) being higher in the case of CA. The water absorption capacity of CA (∼64 %) was almost two folds compared to A (∼31 %) with delayed digestion in the presence of gastric simulated juice with or without pepsin. Both the scaffolds showed the capability to adhere and proliferate muscle satellite cells as single cell culture and muscle satellite along with NIH/3T3 fibroblast cells as co-culture. However, the CA scaffolds showed enhanced capability to adhere and proliferate the two cell lines on its surface compared to A. This work demonstrates an efficient way to fabricate decellularized plant scaffolds with high potential to be used in the production of cultured meat for the food industry.

目前正在做出大量努力来应对当前与传统畜牧业相关的可持续性和环境影响的挑战。这导致了食品创新的进步，指导了基于培养肉的可食用支架的制造。目前的研究工作旨在开发和验证一种新方法，用于制造涂有明胶/海藻酸盐聚合物混合物的脱细胞苹果 3D 多孔支架，用于培养肉生产。制造的无涂层 (A) 和涂层 (CA) 3D 支架呈现出不同的孔径比例，其中中等尺寸的孔径 (100–250 µm) 在 CA 的情况下更高。 CA (~64%) 的吸水能力几乎是 A (~31%) 的两倍，在有或没有胃蛋白酶的模拟胃液存在下消化延迟。两种支架都显示出作为单细胞培养物的肌肉卫星细胞的粘附和增殖能力，以及作为共培养物的肌肉卫星细胞和 NIH/3T3 成纤维细胞的粘附和增殖能力。然而，与 A 相比，CA 支架在其表面粘附和增殖两种细胞系的能力增强。这项工作展示了一种制造脱细胞植物支架的有效方法，具有很高的潜力用于食品工业培养肉的生产。