Electrophoretic deposition of Ru(bpy)32+ in vertically-ordered silica nanochannels: A solid-state electrochemiluminescence sensor for prolidase assay,Biosensors and Bioelectronics

当前位置： X-MOL 学术 › Biosens. Bioelectron. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Electrophoretic deposition of Ru(bpy)32+ in vertically-ordered silica nanochannels: A solid-state electrochemiluminescence sensor for prolidase assay
Biosensors and Bioelectronics ( IF 10.7 ) Pub Date : 2023-12-22 , DOI: 10.1016/j.bios.2023.115967
Weiqiang Yang ₁ , Jiajing Xu ₁ , Qingda Yao ₁ , Xiaoguang Xu ₂ , Xiaoping Chen ₁ , Jiancong Ni ₁ , Qingxiang Wang ₁ , Zhenyu Lin ₃

Affiliation

Prolidase (PLD) plays a crucial role as a dipeptidase in various physiological processes, specifically involved in the cleavage of proline-containing dipeptides for efficient recycling of proline. The accurate determination of PLD activity holds significant importance in clinical diagnosis. Herein, a solid-state electrochemiluminescence (ECL) biosensor was developed to address the urgent need for PLD assay. The Ru(bpy) was electrophoretically deposited within the nanochannels of vertically-ordered mesoporous silica film (VMSF) on indium tin oxide (ITO) electrodes. The Ru(bpy)-deposited VMSF/ITO (Ru-VMSF/ITO) exhibited a remarkable ECL response towards proline, attributed to the enhanced concentration of the reactants and improved electron transfer resulting from the nanoconfinement effect. As PLD specifically enzymolyzed the Gly-Pro dipeptide to release proline, a proline-mediated biosensor was developed for PLD assay. Increased PLD activity led to enhanced release of proline into the porous solid-state ECL sensors, resulting in a more robust ECL signal. There was a linear relationship between ECL intensity and logarithmic concentration of PLD in the range of 10–10000 U/L, with a detection limit of 1.98 U/L. Practical tests demonstrated the reliability and convenience of the proposed bioassay, making it suitable for widespread application in PLD assays.

中文翻译：

Ru(bpy)32+ 在垂直有序二氧化硅纳米通道中的电泳沉积：用于脯氨酸酶测定的固态电化学发光传感器

脯氨酸酶（PLD）作为二肽酶在各种生理过程中发挥着至关重要的作用，特别是参与含脯氨酸二肽的裂解以实现脯氨酸的有效回收。 PLD活性的准确测定在临床诊断中具有重要意义。本文开发了一种固态电化学发光（ECL）生物传感器来解决 PLD 检测的迫切需求。 Ru(bpy) 通过电泳沉积在氧化铟锡 (ITO) 电极上垂直有序介孔二氧化硅薄膜 (VMSF) 的纳米通道内。 Ru(bpy)沉积的VMSF/ITO (Ru-VMSF/ITO)对脯氨酸表现出显着的ECL响应，这归因于反应物浓度的增加和纳米限制效应导致的电子转移的改善。由于 PLD 特异性酶解 Gly-Pro 二肽以释放脯氨酸，因此开发了用于 PLD 测定的脯氨酸介导的生物传感器。 PLD 活性的增加导致脯氨酸向多孔固态 ECL 传感器的释放增加，从而产生更稳定的 ECL 信号。 ECL强度与PLD对数浓度在10~10000 U/L范围内呈线性关系，检测限为1.98 U/L。实际测试证明了所提出的生物测定的可靠性和便利性，使其适合在PLD测定中广泛应用。

更新日期：2023-12-22

点击分享查看原文

点击收藏

阅读更多本刊新发论文