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A facile fluorescence Eu MOF sensor for ascorbic acid and ascorbate oxidase detection
Analytical Methods ( IF 2.7 ) Pub Date : 2023-12-22 , DOI: 10.1039/d3ay01978k Xin-Xin Dong 1 , Tao-Li Chen 1 , Xiang-Juan Kong 1 , Shuang Wu 2 , Fang-Fang Kong 1 , Qiang Xiao 1
Analytical Methods ( IF 2.7 ) Pub Date : 2023-12-22 , DOI: 10.1039/d3ay01978k Xin-Xin Dong 1 , Tao-Li Chen 1 , Xiang-Juan Kong 1 , Shuang Wu 2 , Fang-Fang Kong 1 , Qiang Xiao 1
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In this work, a facile fluorescence Eu3+-based metal–organic framework (Eu MOF) sensor for ascorbic acid (AA) and ascorbate oxidase (AAO) detection was developed. The fluorescence of the Eu MOF could be effectively quenched by Ce3+ but not by Ce4+ at an appropriate concentration, and thus, when the reductant AA was added into the solution containing Ce4+, Ce4+ was chemically reduced to Ce3+, which induced the decreased fluorescence signal of the Eu MOF. However, when AAO was introduced, AA was effectively oxidized to dehydroascorbic acid (DHAA) under the catalysis of AAO, and thus, Ce4+ could not be reduced, resulting in the fluorescence restoration of the Eu MOF. Hence, the concentration of AA and AAO could be determined by the fluorescence decrease and restoration of the Eu MOF. The fluorescent platform showed high sensitivity with a limit of detection of 0.32 μM for AA and 1.18 U L−1 for AAO, respectively. Moreover, the proposed method was successfully applied for AA and AAO determination in real samples, indicating great potential for biomedical application in complex matrices.
中文翻译：
一种用于抗坏血酸和抗坏血酸氧化酶检测的简便荧光 Eu MOF 传感器
在这项工作中，开发了一种用于抗坏血酸（AA）和抗坏血酸氧化酶（AAO）检测的简便荧光Eu 3+基金属有机骨架（Eu MOF）传感器。 Eu MOF的荧光可以被Ce 3+有效猝灭，但不能被适当浓度的Ce 4+猝灭，因此，当将还原剂AA加入到含有Ce 4+的溶液中时，Ce 4+被化学还原为Ce 3+ ，导致Eu MOF的荧光信号减弱。然而，当引入AAO时，AA在AAO的催化下被有效氧化为脱氢抗坏血酸(DHAA)，因此Ce 4+无法被还原，从而导致Eu MOF的荧光恢复。因此，AA和AAO的浓度可以通过Eu MOF的荧光减弱和恢复来确定。荧光平台表现出高灵敏度，AA 的检测限分别为 0.32 μM，AAO 的检测限分别为 1.18 UL -1 。此外，该方法成功应用于实际样品中AA和AAO的测定，表明在复杂基质的生物医学应用中具有巨大潜力。
更新日期：2023-12-22
中文翻译：
一种用于抗坏血酸和抗坏血酸氧化酶检测的简便荧光 Eu MOF 传感器
在这项工作中，开发了一种用于抗坏血酸（AA）和抗坏血酸氧化酶（AAO）检测的简便荧光Eu 3+基金属有机骨架（Eu MOF）传感器。 Eu MOF的荧光可以被Ce 3+有效猝灭，但不能被适当浓度的Ce 4+猝灭，因此，当将还原剂AA加入到含有Ce 4+的溶液中时，Ce 4+被化学还原为Ce 3+ ，导致Eu MOF的荧光信号减弱。然而，当引入AAO时，AA在AAO的催化下被有效氧化为脱氢抗坏血酸(DHAA)，因此Ce 4+无法被还原，从而导致Eu MOF的荧光恢复。因此，AA和AAO的浓度可以通过Eu MOF的荧光减弱和恢复来确定。荧光平台表现出高灵敏度，AA 的检测限分别为 0.32 μM，AAO 的检测限分别为 1.18 UL -1 。此外，该方法成功应用于实际样品中AA和AAO的测定，表明在复杂基质的生物医学应用中具有巨大潜力。
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