Detection of acrylamide in food based on MIL-glucose oxidase cascade colorimetric aptasensor,Analytica Chimica Acta

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Detection of acrylamide in food based on MIL-glucose oxidase cascade colorimetric aptasensor
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2023-12-19 , DOI: 10.1016/j.aca.2023.342150
Kaixi Guo ₁ , Xianfeng Lin ₁ , Nuo Duan ₁ , Chunxia Lu ₂ , Zhouping Wang ₁ , Shijia Wu ₁

Affiliation

Background

Maillard reaction involves the polymerization, condensation, and other reactions between compounds containing free amino groups and reducing sugars or carbonyl compounds during heat processing. This process endows unique flavors and colors to food, while it can also produce numerous hazards. Acrylamide (AAm) is one of Maillard's hazards with neurotoxicity and carcinogenicity, these effects can trigger mutations and alternations in gene expression in human cells and accelerate organ aging. An accurate and reliable acrylamide detection method with high sensitivity and specificity for future regulatory activities is urgently needed.

Results

Herein, we constructed a colorimetric aptasensor with the hybridization of MIL-glucose oxidase (MGzyme)-cDNA and magnetic nanoparticle-aptamer (MNP-Apt) to specifically detect AAm. The incorporation of MB-Apt and AAm released MGzyme-cDNA in the supernatant, took the supernatant out, with the addition of glucose and TMB, MGzyme would oxidize glucose, the resulting •OH facilitated the oxidation of colorless TMB to blue ox-TMB. The absorbance value at 652 nm, which indicates the characteristic absorption peak of ox-TMB, exhibited a proportion to the concentration of AAm. MGzyme avoided the addition of harmful intermediate H₂O₂ and created an acid microenvironment for the catalytic reaction. MNP-Apt possessed the advantages of high specificity and simplified separation. Under optimal conditions, this method displayed a linear range of 0.01–100 μM with the limit of detection of 1.53 nM. With the spiked analysis data cross-verified by ELISA kit, this aptasensor was proven to specifically detect AAm at low concentrations.

Significance

This colorimetric aptasensor was the integration of aptamer and the enzyme-cascade system, which could broaden the applicable range of enzyme-cascade system, break the limits of specific detection of substrates, eliminate the need for harmful intermediates, improve the reaction efficiency, implement the specific detection, whilst enabling the accurate detection of AAm. Given these remarkable performances, this method has shown significant potential in the field of food safety inspection.

中文翻译：

基于MIL-葡萄糖氧化酶级联比色适体传感器检测食品中丙烯酰胺

背景

美拉德反应是指含有游离氨基的化合物与还原糖或羰基化合物在热处理过程中发生的聚合、缩合等反应。这一过程赋予食品独特的风味和颜色，同时也会产生许多危害。丙烯酰胺(AAm)是美拉德危害之一，具有神经毒性和致癌性，这些作用可引发人体细胞基因表达的突变和改变，加速器官衰老。未来的监管活动迫切需要一种准确可靠、具有高灵敏度和特异性的丙烯酰胺检测方法。

结果

在此，我们通过 MIL-葡萄糖氧化酶 (MGzyme)-cDNA 和磁性纳米颗粒-适体 (MNP-Apt) 的杂交构建了比色适体传感器来特异性检测 AAm。 MB-Apt和AAm的掺入在上清液中释放出MGzyme-cDNA，取出上清液，加入葡萄糖和TMB，MGzyme氧化葡萄糖，生成·OH，促进无色TMB氧化为蓝色ox-TMB。 652 nm处的吸光度值表示ox-TMB的特征吸收峰，与AAm的浓度成比例。 MGzyme避免了有害中间体H ₂ O ₂的添加，并为催化反应创造了酸性微环境。 MNP-Apt具有特异性高、分离简单等优点。在最佳条件下，该方法的线性范围为 0.01–100 μM，检测限为 1.53 nM。通过 ELISA 试剂盒交叉验证加标分析数据，证明该适体传感器可以特异性检测低浓度的 AAm。