Phytochelatin-Mediated Cultivar-Dependent Cd Accumulations of Lactuca sativa and Implication for Cd Pollution-Safe Cultivars Screening,Journal of Agricultural and Food Chemistry

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Phytochelatin-Mediated Cultivar-Dependent Cd Accumulations of Lactuca sativa and Implication for Cd Pollution-Safe Cultivars Screening
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-12-20 , DOI: 10.1021/acs.jafc.3c05476
Chun-Tao He _{1,

2} , Xue-Song Wang ₃ , Xia-Xin Hu ₁ , Ju Yuan ₁ , Qian-Hui Zhang ₁ , Xuan-Tong Tan ₁ , Yun-Fan Wang ₃ , Xiao Tan ₄ , Zhong-Yi Yang ₂

Affiliation

Cd pollution-safe cultivar (Cd-PSC) is a feasible strategy to minimize Cd contamination in leafy vegetables. The shoot Cd concentrations of 23 Lactuca sativa cultivars under Cd stress ranged from 0.124 to 2.155 mg·kg^–1 with a maximum cultivar difference of 8 folds. Typical Cd-PSC C16 (L) and high-Cd-accumulating cultivar C13 (H) were screened to investigate the mechanisms of Cd accumulations in L. sativa through determining Cd concentrations, Cd subcellular distributions, phytochelatin profiles, and phytochelatin biosynthesis-related genes’ expressions. Higher Cd distribution in a heat stable fraction in C13 (H) indicated that the high Cd accumulation trait of C13 (H) mainly depended on the Cd-phytochelatin complexes. Root phytochelatin concentrations were significantly elevated in C13 (H) (5.83 folds) than in C16 (L) (2.69 folds) (p < 0.05) under Cd stress. Significantly downregulated expressions of glutathione S-transferase rather than the regulation of phytochelatin synthesis genes in the root of C13 (H) might be responsible for sufficient glutathione supply for phytochelatins synthesis. These findings suggested that phytochelatin elevation in C13 (H) would favor the Cd root to shoot transportation, which provides new insights into the phytochelatin-related cultivar-dependent Cd accumulating characteristic in L. sativa.

中文翻译：

植物螯合素介导的莴苣品种依赖性镉积累及其对镉污染安全品种筛选的意义

镉污染安全品种（Cd-PSC）是最大限度减少叶类蔬菜镉污染的可行策略。镉胁迫下23个莴苣品种地上部镉浓度范围为0.124~2.155 mg·kg ^–1 ，品种间最大差异达8倍。筛选典型Cd-PSC C16（L）和高Cd积累品种C13（H），通过测定Cd浓度、Cd亚细胞分布、植物螯合素谱和植物螯合素生物合成相关基因，研究苜蓿Cd积累机制。 ' 的表达方式。 C13（H）的热稳定部分中较高的Cd分布表明C13（H）的高Cd积累特性主要取决于Cd-植物螯合素复合物。在 Cd 胁迫下，C13 (H) 根部植物螯合素浓度（5.83 倍）显着高于 C16 (L)（2.69 倍）（ p < 0.05）。 C13 (H) 根中谷胱甘肽 S-转移酶的表达显着下调，而不是植物螯合素合成基因的调节，可能是为植物螯合素合成提供充足谷胱甘肽的原因。这些发现表明，C13 (H) 中植物螯合素的升高有利于镉根向地上部的运输，这为了解苜蓿植物螯合素相关的品种依赖性镉积累特征提供了新的见解。

更新日期：2023-12-20

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