A genosensor based on TiO₂-adenine nanocomposites (NCs) modified screen-printed gold electrode surface (TA-SPGE) was immobilized with haemagglutinin (HA) gene specific probe containing 5′ amine group and investigated for swine flu virus (H1N1). Bare SPGE surface was modified by drop coating of the nanocomposites. The NCs and precursors were characterized structurally and morphologically by adopting various techniques such as Fourier Transform Infrared (FTIR) spectra, X-ray Diffraction (XRD) spectra, UV–Visible spectra, Raman spectra, Zeta sizer, Field-Emission Scanning Electron Microscopy (FE-SEM), Energy Dispersive spectra (EDS) and High-Resolution Transmission Electron Microscopy (HR-TEM) with Selected Area Electron Diffraction (SAED) pattern. Electrochemical techniques termed as cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectra (EIS) were employed for analytical detection of single-stranded viral genome in phosphate buffer saline (pH-7.2) by using methylene blue (MB) redox indicator. Moreover, the performance of designed electrode was optimized at different concentration, pH, and scan rates. The proposed genosensor revealed high sensitivity 10.6 μA/ng.cm², lower detection limit (LOD) of 6.7 × 10^-8 ng/mL and wide linear range of 3 × 10^-8-3 × 10^-3 ng/mL with regression coefficient of R² = 0.989 towards swine flu virus DNA. Additionally, the modified genosensor revealed good stability and specificity against human DNA and various pathogens such as H3N2 influenza virus, Bacillus subtilis, Neisseria meningitidis, Staphylococcus aureus and Streptococcus pyogenes. The proposed genosensor is based on relatively simple methodology, quite amicable and could promptly be used as point-of-care test for swine flu virus detection.

基于TiO ₂ -腺嘌呤纳米复合材料（NCs）修饰的丝网印刷金电极表面（TA-SPGE）的基因传感器固定有含有5'胺基团的血凝素（HA）基因特异性探针，并研究了猪流感病毒（H1N1）。通过纳米复合材料的滴涂来修饰裸 SPGE 表面。采用傅里叶变换红外（FTIR）光谱、X射线衍射（XRD）光谱、紫外-可见光谱、拉曼光谱、Zeta粒度仪、场发射扫描电子显微镜等多种技术对NC和前体进行结构和形态表征。 FE-SEM）、能量色散光谱（EDS）和具有选区电子衍射（SAED）模式的高分辨率透射电子显微镜（HR-TEM）。采用循环伏安法（CV）、微分脉冲伏安法（DPV）和电化学阻抗谱（EIS）等电化学技术，使用亚甲蓝（MB）对磷酸盐缓冲液（pH-7.2）中的单链病毒基因组进行分析检测氧化还原指示剂。此外，设计的电极的性能在不同浓度、pH 和扫描速率下进行了优化。所提出的基因传感器具有10.6 μA/ng.cm ^{2的高灵敏度、6.7 × 10 -8} ng/mL的检测下限 (LOD)和 3 × 10 ^-8 -3 × 10 ^-3 ng/mL的宽线性回归范围 猪流感病毒DNA的系数R ^{2 = 0.989。}此外，改进的基因传感器对人类DNA和各种病原体（例如H3N2流感病毒、枯草芽孢杆菌、脑膜炎奈瑟菌、金黄色葡萄球菌和化脓性链球菌）表现出良好的稳定性和特异性。所提出的基因传感器基于相对简单的方法，非常友好，可以立即用作猪流感病毒检测的即时测试。