Psoriasis vulgaris is the most common form of the four clinical types. However, early diagnosis of psoriasis vulgaris is difficult due to the lack of effective biomarkers. The aim of this study was to screen potential biomarkers for the diagnosis of psoriasis. In our study, we downloaded the original data from GSE30999 and GSE41664, and the autophagy-related genes list from human autophagy database to identify differentially expressed autophagy-related genes (DERAGs) by R software. Then Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed for DERAGs. DERAGs were validated by the other four databases (GSE13355, GSE14905, GSE6710, and GSE55201) to screen biomarkers with high diagnostic value for the early diagnosis of psoriasis vulgaris. Finally, DERAGs were verified in our clinical blood samples by ELISA. A total of 12 DERAGs were identified between 123 paired non-lesional and lesional skin samples from patients with psoriasis vulgaris. GO and KEGG enrichment analysis indicated the TORC2 complex was more enriched and the NOD-like receptor signaling pathway was mostly enriched. Three autophagy-related genes (BIRC5, NAMPT and BCL2) were identified through bioinformatics analysis and verified by ELISA in clinical blood samples. And these genes showed high diagnostic value for the early diagnosis of psoriasis vulgaris. We identified three autophagy-related genes (BIRC5, NAMPT and BCL2) with high diagnostic value for the early diagnosis of psoriasis vulgaris through bioinformatics analysis and clinical samples. Therefore, we proposed that BIRC5, NAMPT and BCL2 may be as potential biomarkers for the early diagnosis of psoriasis vulgaris. In addition, BIRC5, NAMPT and BCL2 may affect the development of psoriasis by regulating autophagy.

寻常型银屑病是四种临床类型中最常见的形式。然而，由于缺乏有效的生物标志物，寻常型银屑病的早期诊断很困难。本研究的目的是筛选诊断牛皮癣的潜在生物标志物。在我们的研究中，我们下载了GSE30999和GSE41664的原始数据，以及人类自噬数据库中的自噬相关基因列表，通过R软件识别差异表达的自噬相关基因（DERAG）。然后对 DERAG 进行基因本体论 (GO) 和京都基因和基因组百科全书 (KEGG) 通路富集分析。 DERAG经过其他四个数据库（GSE13355、GSE14905、GSE6710和GSE55201）的验证，筛选出对寻常型银屑病早期诊断具有高诊断价值的生物标志物。最后，通过 ELISA 在我们的临床血液样本中验证了 DERAG。在来自寻常型银屑病患者的 123 个配对的非病变和病变皮肤样本中总共鉴定出了 12 个 DERAG。 GO和KEGG富集分析表明TORC2复合物较为富集，NOD样受体信号通路也大部分富集。通过生物信息学分析鉴定出三个自噬相关基因（ BIRC5 、 NAMPT和BCL2 ），并通过ELISA在临床血液样本中进行验证。并且这些基因对于寻常型银屑病的早期诊断显示出很高的诊断价值。我们通过生物信息学分析和临床样本，鉴定出三个对寻常型银屑病早期诊断具有较高诊断价值的自噬相关基因（ BIRC5 、 NAMPT和BCL2 ）。 因此，我们提出BIRC5 、 NAMPT和BCL2可能作为寻常型银屑病早期诊断的潜在生物标志物。此外， BIRC5 、 NAMPT和BCL2可能通过调节自噬影响银屑病的发生发展。