This research examines the biological treatment of undiluted vegetable waste digestate conducted in a bubble column photobioreactor. Initially, the bioreactor containing 3N-BBM medium was inoculated with Microglena sp., Tetradesmus obliquus, and Desmodesmus subspicatus mixture with a density of 1.0 × 10⁴ cells/mL and the consortium was cultivated for 30 days. Then, the bioreactor was semi-continuously fed with liquid digestate with hydraulic retention time (HRT) of 30 days, and the treatment process was continued for the next 15 weeks. The change in the microalgal and cyanobacterial species domination was measured in regular intervals using cell counting with droplet method on a microscope slide. At the end of the experiment, Desmonostoc sp. cyanobacteria (identified with 16S ribosomal RNA genetical analysis) as well as Tetradesmus obliquus green algae along with Rhodanobacteraceae and Planococcaceae bacteria (determined with V3–V4 16sRNA metagenomic studies) dominated the microbial community in the photobioreactor. The experiment demonstrated high treatment efficiency, since nitrogen and soluble COD were removed by 89.3 ± 0.5% and 91.2 ± 1.6%, respectively, whereas for phosphates, 72.8 ± 2.1% removal rate was achieved.

这项研究研究了在泡罩塔光生物反应器中对未稀释的蔬菜废物消化物进行的生物处理。最初，以1.0×10 ^{4 个}细胞/mL的密度将Microglena sp.、 Tetradesmus obliquus和Desmodesmus subspicatus混合物接种到含有3N-BBM培养基的生物反应器中，并将该聚生体培养30天。然后，向生物反应器半连续地注入液体消化物，水力停留时间（HRT）为30天，处理过程持续接下来的15周。使用显微镜载玻片上的液滴法进行细胞计数，定期测量微藻和蓝藻物种优势的变化。在实验结束时， Desmonostoc sp。蓝细菌（通过 16S 核糖体 RNA 遗传分析鉴定）以及斜叶绿藻以及红细菌科和Planococcaceae细菌（通过 V3-V4 16sRNA 宏基因组研究确定）在光生物反应器中主导微生物群落。实验证明了较高的处理效率，氮和可溶性 COD 的去除率分别为 89.3 ± 0.5% 和 91.2 ± 1.6%，而磷酸盐的去除率为 72.8 ± 2.1%。