Enhanced sludge hydrolysis in micro-aerobic environment with dissolved oxygen (DO) regulation could degrade stubborn refractory N, N-dimethylformamide (DMF) in membrane-making wastewater. However, investigations on how micro-aerobic condition affects DMF conversion and microbial metabolism in biological system are still inadequate. Herein, the responses of microbial community to micro-aerobic environment, and the consequent variations of metabolic pathways in DMF degradation were unraveled. Results indicated that low concentration of DO (0.2–0.4 mg/L) could significantly enhance the ammonification rate (from 21.8 % to 52.8 %) and COD removal efficiency (from 65.2 % to 82 %) by breaking C–N and CO bonds of amide group in macromolecular organics, which help it degraded into micromolecular organics. 16S rRNA and metagenomic analysis further revealed that low DO contents prompted the changes of microbial community structure. Specifically, toxicity-tolerant fermentation bacteria (e.g. Desulfomicrobium and unclassified_c_ Deltaproteobacteria) gradually became the dominant species in micro-aerobic environment, showing positive connections with COD removal and ammonification functions. Additionally, functional genes analysis further revealed the intracellular metabolic pathways of DMF, where the upregulation of tricarboxylic acid (TCA) cycle and oxidative phosphorylation pathway was observed in micro-aerobic environment. Such regulation led to the massive synthesis of adenosine triphosphate (ATP) and NADH, which are needful in oxidative demethylation process, and thus promoting the conversion from DMF to formamide, CH₄ and NH₃ in Methanolobus step-by-step. Therefore, this study provided vital insights into the responses of microbial community structure and DMF intracellular metabolic pathways to micro-aerobic environment, and profoundly discussed their contributions to the efficient treatment effect on membrane-making wastewater.

通过调节溶解氧 (DO) 增强微好氧环境中的污泥水解可降解顽固的难降解物质N, N-二甲基甲酰胺 (DMF)在制膜废水中。然而，关于微需氧条件如何影响生物系统中DMF转化和微生物代谢的研究仍然不足。在此，揭示了微生物群落对微需氧环境的反应，以及DMF降解中代谢途径的相应变化。结果表明，低浓度DO（0.2~0.4mg/L）可通过破坏C-N和C，显着提高氨化率（从21.8%提高到52.8%）和COD去除率（从65.2%提高到82%）₄）逐渐成为微好氧环境中的优势物种，与COD去除和氨化功能呈正相关。此外，功能基因分析进一步揭示了DMF的细胞内代谢途径，在微氧环境中观察到三羧酸（TCA）循环和氧化磷酸化途径的上调。这种调控导致氧化去甲基化过程所需的三磷酸腺苷 (ATP) 和 NADH 大量合成，从而促进 DMF 转化为甲酰胺 CHunclassified_c_ Deltaproteobacteria和Desulfomicrobium大分子有机物中酰胺基的O键，有助于其降解为小分子有机物。 16S rRNA和宏基因组分析进一步揭示低DO含量促使微生物群落结构的变化。具体来说，耐毒发酵细菌（例如Methanolobus 中的 a i=9> 和 NH₃ 。因此，本研究为微生物群落结构和DMF胞内代谢途径对微好氧环境的响应提供了重要的见解，并深入探讨了它们对膜制废水高效处理效果的贡献。