Amorphophallus konjac, belonging to the genus Amorphophallus of the Araceae family, is an economically important crop widely used in health products and biomaterials. In the present work, we performed the whole-genome assembly of A. konjac based on the NovaSeq platform sequence data. The final genome assembly was 4.58 Gb with a scaffold N50 of 3212 bp. The genome includes 39,421 protein-coding genes, and 71.75% of the assemblies were repetitive sequences. Comparative genomic analysis showed 1647 gene families have expanded and 2685 contracted in the A. konjac genome. Likewise, genome evolution analysis indicated that A. konjac underwent whole-genome duplication, possibly contributing to the expansion of certain gene families. Furthermore, we identified many candidate genes involved in the tuber formation and development, cellulose and lignification synthesis. The genome of A. konjac obtained in this work provides a valuable resource for the further study of the genetics, genomics, and breeding of this economically important crop, as well as for evolutionary studies of Araceae family.

魔芋属于天南星科魔芋属，是一种重要的经济作物，广泛应用于保健品和生物材料。在本工作中，我们基于NovaSeq平台序列数据进行了魔芋全基因组组装。最终基因组组装大小为 4.58 Gb，支架 N50 为 3212 bp。基因组包括39,421个蛋白质编码基因，其中71.75%的组装是重复序列。比较基因组分析显示，魔芋基因组中有 1647 个基因家族扩增，2685 个基因家族收缩。同样，基因组进化分析表明魔芋经历了全基因组复制，可能有助于某些基因家族的扩展。此外，我们还鉴定了许多参与块茎形成和发育、纤维素和木质化合成的候选基因。本工作获得的魔芋基因组为进一步研究这种重要经济作物的遗传学、基因组学和育种以及天南星科的进化研究提供了宝贵的资源。