<br>使用光热免疫传感器诊断临床样本中被忽视的热带人畜共患病、钩端螺旋体病,Analytical Chemistry

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使用光热免疫传感器诊断临床样本中被忽视的热带人畜共患病、钩端螺旋体病
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-12-19 , DOI: 10.1021/acs.analchem.3c04447
Kannan Sapna _{1,

2} , Yoon-Bo Shim ₃ , Ananthapadmanabha Bhagwath Arun ₂ , Kariate Sudhakara Prasad _{1,

4}

Affiliation

基于纳米材料的光热生物传感因其普遍性和简单性而受到越来越多的关注。资源匮乏地区被忽视的热带病 (NTD) 的诊断在速度、准确性和成本效益方面都面临着挑战。通过利用碳纳米管 (CNT) 的光热特性，可以使用简单的温度测量来生成定量的生化读数。本文通过简单温度计对目标生物标志物LipL32的灵敏监测，建立了基于CNT标记单克隆抗体的钩端螺旋体病检测光热免疫传感器。在最佳条件下，线性范围高达 10 ⁶ pg/mL，检测限 (LOD) 为 300 fg/mL。总体而言，所提出的免疫测定法表现出良好的精度、选择性和可接受的稳定性。使用光热传感器进行的临床患者样本分析证明了钩端螺旋体病与其他发热性疾病的鉴别诊断。另一方面，我们还利用表面形态和光谱技术对光热传感器平台进行了表征，以确认免疫传感器的稳健和成功制造。所制造的光热传感器可以用作潜在的诊断工具，用于在资源有限的环境中早期检测患者的 NTD，因为它不需要样品预处理、复杂的设备或熟练的劳动力。此外，开发的光热测定遵循 ASSURED 标准，这对于资源有限环境中的诊断非常重要。

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Diagnosis of Neglected Tropical Zoonotic Disease, Leptospirosis in a Clinical Sample Using a Photothermal Immunosensor

Photothermal biosensing based on nanomaterials has gained increasing attention because of its universality and simplicity. Diagnostics of neglected tropical diseases (NTDs) in low-resource settings are challenging in terms of speed, accuracy, and cost-effectiveness. By exploiting the photothermal property of carbon nanotubes (CNTs), simple thermometric measurements can be used to generate quantitative biochemical readouts. Herein, a photothermal immunosensor for leptospirosis detection based on a CNT-labeled monoclonal antibody is established through the sensitive monitoring of the target biomarker LipL32 with a simple thermometer. Under optimum conditions, a linear range up to 10⁶ pg/mL with a limit of detection (LOD) of 300 fg/mL was obtained. Overall, the proposed immunoassay exhibited good precision, selectivity, and acceptable stability. Clinical patient sample analysis with the photothermal sensor proved the differential diagnosis of leptospirosis along with other febrile illnesses. On the other hand, we have also characterized the photothermal sensor platform with surface morphological and spectral techniques to confirm the robust and successful fabrication of the immunosensor. The fabricated photothermal sensor could be used as a potential diagnostic tool for the early detection of NTDs in patients from resource-limited settings, as it does not require sample pretreatment, sophisticated equipment, or skilled labor. Moreover, the developed photothermal assay follows ASSURED criteria, very crucial for diagnosis in resource-limited settings.

更新日期：2023-12-19

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